Catalog Number: 1305
Chemical Name: 1,2,3,4-Tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-5-pyrimidinecarboxylic acid, ethyl ester
Biological Activity
Potent, cell-permeable, small molecule mitosis inhibitor that does not interact with tubulin. Arrests cells in mitosis and specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein required for mitotic spindle formation and maintenance (IC50 = 14 μM).
Technical Data
  • M.Wt:
    292.35
  • Formula:
    C14H16N2O3S
  • Solubility:
    Soluble to 20 mM in ethanol and to 100 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Store at RT
  • CAS No:
    254753-54-3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Licensing Caveats:
Sold with the permission of Harvard University
Background References
  1. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5.
    Kapoor et al.
    J.Cell Biol., 2000;150:975
  2. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen.
    Mayer et al.
    Science, 1999;286:971
Citations:

The citations listed below are publications that use Tocris products. Selected citations for Monastrol include:

11 Citations: Showing 1 - 10
Filter your results:

  1. Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.
    Authors: Shrestha
    Nat Commun 2017;8(1):150
  2. C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle.
    Authors: Floriot Et al.
    Cell Cycle 2015;6:6894
  3. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
    Authors: Barr and Bakal
    Nat Commun 2015;5:10564
  4. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation.
    Authors: Kabeche and Compton
    J Biol Chem 2013;502:110
  5. p31(comet) inactivates the chemically induced Mad2-dependent spindle assembly checkpoint and leads to resistance to anti-mitotic drugs.
    Authors: Habu and Matsumoto
    Nature 2013;2:562
  6. Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
    Authors: Shrestha and Draviam
    Curr Biol 2013;23:1514
  7. Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance.
    Authors: Corrigan Et al.
    Springerplus 2013;12:2643
  8. High mitotic activity of Polo-like kinase 1 is required for chromosome segregation and genomic integrity in human epithelial cells.
    Authors: Lera and Burkard
    J Biol Chem 2012;287:42812
  9. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors.
    Authors: Bassett Et al.
    Sci Rep 2010;190:177
  10. Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation.
    Authors: Samoshkin Et al.
    PLoS One 2009;4:e6831
  11. Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle.
    Authors: Trinkle-Mulcahy Et al.
    Mol Biol Cell 2003;14:107
Expand to show all 11 Citations

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