Monoamine Oxidase B Antibody (5M8A5)

Novus Biologicals | Catalog # NBP3-15411

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5M8A5 expressed in HEK293
View all Monoamine Oxidase B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338). MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Monoamine Oxidase B Antibody (5M8A5)

Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Mouse testis using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Rat lung using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411]

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Human colon using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Monoamine Oxidase B Antibody (5M8A5)

Simple Western: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] -

Simple Western: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Monoamine Oxidase B Antibodies (NBP3-15411), ProteinSimple Western Blot on Jess Instrument. 1 microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.
Monoamine Oxidase B Antibody (5M8A5)

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] -

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human liver tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Monoamine Oxidase B Antibody (5M8A5)

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] -

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human tonsil tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Monoamine Oxidase B Antibody (5M8A5)

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] -

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human colon carcinoma tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Monoamine Oxidase B Antibody (5M8A5)

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] -

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human kidney tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for Monoamine Oxidase B Antibody (5M8A5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry

1:50 - 1:200

Simple Western

Validated from a verified customer review.

Western Blot

1:500 - 1:1000
Application Notes

See Simple Western Antibody Database for Simple Western validation

Reviewed Applications

Read 1 review rated 5 using NBP3-15411 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Monoamine Oxidase B

Monoamine Oxidase B is encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.

Long Name

Amine oxidase [flavin-containing] B

Alternate Names

EC 1.4.3.21, EC 1.4.3.4, MAO-B, MAOB

Gene Symbol

MAOB

Additional Monoamine Oxidase B Products

Product Documents for Monoamine Oxidase B Antibody (5M8A5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Monoamine Oxidase B Antibody (5M8A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Monoamine Oxidase B Antibody (5M8A5) (1)

5 out of 5
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  • Monoamine Oxidase B Antibody (5M8A5)
    Name: Stella Dracheva
    Application: Simple Western
    Sample Tested: Human Brain Tissue
    Species: Human
    Verified Customer | Posted 04/06/2023
    MAOB Antibodies NBP3-15411, ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times.
    Monoamine Oxidase B Antibody (5M8A5) NBP3-15411
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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