Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503

Specificity

Detects mouse 5'‑Nucleotidase/CD73 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse 5'‑Nucleotidase/CD73 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse 5"-Nucleotidase/CD73 (Catalog # 4488-EN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

References

  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Entrez Gene IDs

4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)

Gene Symbol

NT5E

UniProt

Q61503

Additional 5'-Nucleotidase/CD73 Products

Product Documents for Mouse 5'‑Nucleotidase/CD73 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse 5'‑Nucleotidase/CD73 Biotinylated Antibody

For research use only

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