Key Product Details

Species Reactivity

Validated:

Mouse, Porcine

Cited:

Mouse, Rat, Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503

Specificity

Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody

Detection of 5'-Nucleotidase/CD73 antibody in Mouse CD4+Spleno-cytes antibody by Flow Cytometry.

Detection of 5'-Nucleotidase/CD73 in Mouse CD4+Spleno-cytes by Flow Cytometry.

Mouse CD4+splenocytes was stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Detection of 5'-Nucleotidase/CD73 antibody in Porcine Mesenchymal Stem Cells antibody by Flow Cytometry.

Detection of 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells by Flow Cytometry.

Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
5'-Nucleotidase/CD73 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

5'‑Nucleotidase/CD73 in Mouse Kidney.

5'-Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
5'-Nucleotidase/CD73 antibody in Porcine Mesenchymal Stem Cells by Immunocytochemistry (ICC).

5'‑Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells.

5'-Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Mouse 5'-Nucleotidase/CD73 by Immunocytochemistry/Immunofluorescence

Detection of Mouse 5'-Nucleotidase/CD73 by Immunocytochemistry/Immunofluorescence

Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder and left and right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) and uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) and merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse CD4+ splenocytes and porcine mesenchymal stem cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed porcine mesenchymal stem cells

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)

Reviewed Applications

Read 2 reviews rated 4 using AF4488 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

References

  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Entrez Gene IDs

4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)

Gene Symbol

NT5E

UniProt

Q61503

Additional 5'-Nucleotidase/CD73 Products

Product Documents for Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody

For research use only

Citations for Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody

Customer Reviews for Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody (2)

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23678122
    Species: Other
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22219293
    Species: Mouse
    Verified Customer | Posted 01/08/2015

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