Mouse/Porcine 5'-Nucleotidase/CD73 Antibody

Catalog # Availability Size / Price Qty
AF4488
AF4488-SP
Detection of 5'-Nucleotidase/CD73 in Mouse CD4+ Spleno­cytes by Flow Cytometry.
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Citations (2)
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Mouse/Porcine 5'-Nucleotidase/CD73 Antibody Summary

Species Reactivity
Mouse, Porcine
Specificity
Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of 5'-Nucleotidase/CD73 in Mouse CD4+ Spleno­cytes by Flow Cytometry. View Larger

Detection of 5'-Nucleotidase/CD73 in Mouse CD4+ Spleno­cytes by Flow Cytometry. Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Flow Cytometry Detection of 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells by Flow Cytometry. View Larger

Detection of 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Immunohistochemistry 5'‑Nucleotidase/CD73 in Mouse Kidney. View Larger

5'‑Nucleotidase/CD73 in Mouse Kidney.

5'‑Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry 5'‑Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells. View Larger

5'‑Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells. 5'‑Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

References
  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
Entrez Gene IDs
4907 (Human); 23959 (Mouse); 58813 (Rat)
Alternate Names
5' nucleotidase (CD73); 5'-NT; 5-NT; 5'Nucleotidase; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase

Product Datasheets

Citations for Mouse/Porcine 5'-Nucleotidase/CD73 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Molecular and Microarray-based Analysis of Diversity among Resting and Osteogenically Induced Porcine Mesenchymal Stromal Cells of Several Tissue Origin
    Authors: Samet Bayraktar
    J Tissue Eng Regen Med, 2016;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow
  2. Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits.
    Authors: Sowa NA, Taylor-Blake B, Zylka MJ
    J. Neurosci., 2010;30(6):2235-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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