Mouse/Porcine 5'-Nucleotidase/CD73 Antibody

Catalog # Availability Size / Price Qty
AF4488
AF4488-SP

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Detection of 5'-Nucleotidase/CD73 in Mouse CD4+Spleno-cytes by Flow Cytometry.
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Citations (11)
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Mouse/Porcine 5'-Nucleotidase/CD73 Antibody Summary

Species Reactivity
Mouse, Porcine
Specificity
Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of 5'-Nucleotidase/CD73 antibody in Mouse CD4<SUP>+</SUP>Spleno-cytes antibody by Flow Cytometry. View Larger

Detection of 5'-Nucleotidase/CD73 in Mouse CD4+Spleno-cytes by Flow Cytometry. Mouse CD4+splenocytes was stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Flow Cytometry Detection of 5'-Nucleotidase/CD73 antibody in Porcine Mesenchymal Stem Cells antibody by Flow Cytometry. View Larger

Detection of 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Immunohistochemistry 5'-Nucleotidase/CD73 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

5'‑Nucleotidase/CD73 in Mouse Kidney. 5'-Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry 5'-Nucleotidase/CD73 antibody in Porcine Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

5'‑Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells. 5'-Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry/ Immunofluorescence Detection of Mouse 5'-Nucleotidase/CD73 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse 5'-Nucleotidase/CD73 by Immunocytochemistry/Immunofluorescence Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder and left and right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) and uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) and merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

References
  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
Entrez Gene IDs
4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)
Alternate Names
5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase

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Citations for Mouse/Porcine 5'-Nucleotidase/CD73 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosis
    Authors: Busra Isik, Roman Thaler, Busra B. Goksu, Sabena M. Conley, Hayder Al-Khafaji, Arjunmohan Mohan et al.
    Stem Cell Research & Therapy
  2. Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT)
    Authors: Jianying Zhang, Feng Li, Kelly M. Williamson, Susheng Tan, Devon Scott, Kentaro Onishi et al.
    Cell and Tissue Research
  3. Kidney inflammaging is promoted by CCR2+ macrophages and tissue-derived micro-environmental factors
    Authors: Lise Lefèvre, Jason S. Iacovoni, Hélène Martini, Julie Bellière, Damien Maggiorani, Marianne Dutaur et al.
    Cellular and Molecular Life Sciences
  4. Perivascular CD73+ cells attenuate inflammation and interstitial fibrosis in the kidney microenvironment
    Authors: Heather M. Perry, Nicole Görldt, Sun-sang J. Sung, Liping Huang, Kinga P. Rudnicka, Iain M. Encarnacion et al.
    American Journal of Physiology-Renal Physiology
  5. Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss
    Authors: Yu-Ru V. Shih, Mengqian Liu, Seong Keun Kwon, Masayuki Iida, Ya Gong, Nivedita Sangaj et al.
    Science Advances
  6. Molecular and Microarray-based Analysis of Diversity among Resting and Osteogenically Induced Porcine Mesenchymal Stromal Cells of Several Tissue Origin
    Authors: Samet Bayraktar
    J Tissue Eng Regen Med, 2017-05-19;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Expression of Prostatic Acid Phosphatase in Rat Circumvallate Papillae
    Authors: Kentaro Nishida, Teruyo Kubota, Saki Matsumoto, Junki Kato, Yu Watanabe, Atsuko Yamamoto et al.
    PLOS ONE
  8. FACS Array Profiling Identifies Ecto-5′ Nucleotidase as a Striatopallidal Neuron-Specific Gene Involved in Striatal-Dependent Learning
    Authors: Sabrina L. Ena, Jean-François De Backer, Serge N. Schiffmann, Alban de Kerchove d'Exaerde
    The Journal of Neuroscience
  9. Adenosine Enhances Sweet Taste through A2B Receptors in the Taste Bud
    Authors: Robin Dando, Gennady Dvoryanchikov, Elizabeth Pereira, Nirupa Chaudhari, Stephen D. Roper
    The Journal of Neuroscience
  10. Recombinant ecto-5'-nucleotidase (CD73) has long lasting antinociceptive effects that are dependent on adenosine A1 receptor activation
    Authors: Nathaniel A Sowa, Meagen K Voss, Mark J Zylka
    Mol Pain
  11. Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits.
    Authors: Sowa NA, Taylor-Blake B, Zylka MJ
    J. Neurosci., 2010-02-10;30(6):2235-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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