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OR

Mouse/Porcine 5'‑Nucleotidase/CD73 Antibody

  • Specificity
    Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
    Trp29-Lys549
    Accession # Q61503
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of 5'-Nucleotidase/CD73 in Mouse CD4+ Spleno­cytes by Flow Cytometry. Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Immunocytochemistry
5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells. 5'-Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Immunohistochemistry
5'-Nucleotidase/CD73 in Mouse Kidney.

5'-Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

  • References:
    1. Resta, R. et al. (1993) Gene 133:171.
    2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
    3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
  • Entrez Gene IDs:
    23959 (Human); 4907 (Human); 58813 (Rat);
  • Alternate Names:
    5-NT; CD73; E5NT; eNT; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5'-nucleotidase, ecto (CD73); CD73; CD73 antigen; E5NT; EC 3.1.3.5; Ecto-5'-nucleotidase; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NTE; Purine 5-Prime-Nucleotidase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits.
    Authors: Sowa NA, Taylor-Blake B, Zylka MJ
    J. Neurosci., 2010;30(6):2235-44.
    Species: Mouse
    Application: IHC,

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