Rat IgG2A Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
IC006F
Detection of Rat IgG Control by Flow Cytometry
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Product Details
Citations (2)
FAQs
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Rat IgG2A Fluorescein-conjugated Antibody Summary

Specificity
Selected for use as rat IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2A Clone # 54447
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Rat IgG Control by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB17582F, filled histogram) or Rat IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Product Datasheets

Citations for Rat IgG2A Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010;83(3):404-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  2. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2006;36(5):237-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

FAQs

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