Rat IgG2A Fluorescein-conjugated Antibody

(2 citations)   
  • Specificity
    Selected for use as rat IgG2A isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG2A Clone # 54447
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line wasstained with Rat Anti-Mouse Rae-1 Pan Specific Fluorescein-conjugatedMonoclonal Antibody (Catalog # FAB17582F,filled histogram) or Rat IgG Fluorescein-conjugated Isotype Control Antibody(Catalog # IC006F, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010;83(3):404-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  2. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2006;36(5):237-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

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Rat IgG2A PE-conjugated Antibody

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Rat IgG2A Allophycocyanin Isotype Control

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Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Rat IgG2A Alexa Fluor® 647-conjugated Antibody

Ctrl IC006R
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Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Rat IgG2A Biotinylated Isotype Control

Ctrl IC006B
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Rat IgG2A Alexa Fluor® 750-conjugated Antibody

Ctrl IC006S
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Rat IgG2A Alexa Fluor® 594-conjugated Antibody

Ctrl IC006T
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Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Rat IgG2A PerCP‑conjugated Control Antibody

Ctrl IC006C
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