Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all 5T4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse 5T4
Ser30-Ser361
Accession # AAH58198

Specificity

Detects mouse 5T4 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human 5T4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse 5T4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse 5T4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5T4

5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72‑80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30‑361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90‑338). Over aa 30‑361, mouse 5T4 shares 95% and 81% aa identity with rat and human 5T4, respectively.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

AAH58198

Additional 5T4 Products

Product Documents for Mouse 5T4 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse 5T4 Biotinylated Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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