Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all 5T4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse 5T4
Ser30-Ser361
Accession # AAH58198

Specificity

Detects mouse 5T4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human 5T4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse 5T4 Antibody

Detection of 5T4 antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of 5T4 in D3 Mouse Cell Line by Flow Cytometry.

Retinoic acid-treated D3 mouse embryonic stem cell line was stained with Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

5T4 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

5T4 in D3 Mouse Cell Line.

5T4 was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm/membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse 5T4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Retinoic acid‑treated D3 mouse embryonic stem cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse 5T4

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5T4

5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72‑80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30‑361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90‑338). Over aa 30‑361, mouse 5T4 shares 95% and 81% aa identity with rat and human 5T4, respectively.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

AAH58198

Additional 5T4 Products

Product Documents for Mouse 5T4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse 5T4 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Mouse 5T4 Antibody

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Protocols

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