Mouse Bik-like Killer Protein Affinity Purified PAb

Catalog #: AF849 Datasheet / COA / SDS

Discontinued Product

AF849 has been discontinued.
View all Bik-like Killer Protein products.
Detection of Mouse Bik‑like Killer Protein by Western Blot.
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Mouse Bik-like Killer Protein Affinity Purified PAb Summary

Species Reactivity
Mouse
Specificity
Detects mouse BLK.
Source
Polyclonal Rabbit IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Bik‑like Killer Protein antibody by Western Blot. View Larger

Detection of Mouse Bik‑like Killer Protein by Western Blot. Western blot shows lysates ofE. coliexpressing amino acids 1-124 of recombinant mouse BLK. PVDF membrane was probed with 1 µg/mL Mouse Bik‑like Killer Protein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF849) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, lystates of mouse seminal vesicle, kidney, and liver tissue were included. A specific band for Bik‑like Killer Protein was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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