Mouse C-Reactive Protein/CRP Biotinylated Antibody
Mouse C-Reactive Protein/CRP Biotinylated Antibody Summary
Accession # Q91XB3
Mouse C-Reactive Protein/CRP Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Reactive Protein/CRP
C-reactive protein (CRP) is a member of the pentraxin family of plasma proteins that are part of the lectin fold superfamily of calcium-dependent, carbohydrate-binding proteins (1). CRP is named for its ability to bind to the C-polysaccharide of Strep. Pneumoniae. CRP is characterized by cyclic pentameric structure that contains five identical protomers/subunits, each exhibiting a lectin fold composed of two antiparallel beta -sheets with a fattened jellyroll topology. The mouse CRP precursor is 225 amino acids (aa) in length and contains a signal peptide of 19 aa with a mature polypeptide of 206 aa (2, 3). There is one intrachain disulfide bond and no N-linked glycosylation site(s). Although rat CRP is glycosylated at an N-linked site, human, mouse and rabbit CRP all appear to be non-glycosylated (1, 4, 5). In mouse, the protomers are assembled non-covalently to form the pentameter; in rat, two of the five protomers are covalently linked (6). Mature mouse CRP shares 74%, 71%, 79%, and 68% aa sequence identity with rat, human, hamster and guinea pig CRP, respectively. In human, CRP is induced in hepatocytes principally by IL-6 (1). In mouse, IL-6 has very little effect. Mouse CRP induction is due principally to IL-1 (1, 7), with another pentraxin, SAP, being IL-6 inducible (7). CRP exhibits calcium-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP will also bind to the cell membrane of injured necrotic and apoptotic cells. In this context, CRP acts as an opsonin, binding to Fc gamma RI and II, and serves as an anti-inflammatory agent (8).
- Hirschfield, G.M. and M.B. Pepys (2003) Q.J. Med 96:793.
- Whitehead, A.S. et al. (1990) Biochem. J. 266:283.
- Ohnishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 156:814.
- Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
- Black, S. et al. (2004) J. Biol. Chem. 279:48487.
- Rassouli, M. et al. (1992) J. Biol. Chem. 267:2947.
- Ku, N-O. and R.F. Mortensen (1993) Cytokine 5:319.
- Mold, C. et al. (2002) J. Immunol. 169:7019.
Citation for Mouse C-Reactive Protein/CRP Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice.
Authors: Teupser D, Weber O, Rao TN, Sass K, Thiery J, Fehling HJ
J. Biol. Chem., 2011;286(8):6272-9.
Sample Types: Serum
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Mouse C-Reactive Protein/CRP Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Mouse C-Reactive Protein/CRP Biotinylated Antibody and earn rewards!
Have you used Mouse C-Reactive Protein/CRP Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image