Mouse C-Reactive Protein/CRP Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1829
Product Details
Citations (1)
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Mouse C-Reactive Protein/CRP Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse C-Reactive Protein/CRP in Western blots and ELISAs. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human C-Reactive Protein/CRP, recombinant rat C-Reactive Protein/CRP and recombinant porcine C-Reactive Protein/CRP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse C-Reactive Protein/CRP (R&D Systems, Catalog # 1829-CR)
His20-Ser225
Accession # Q91XB3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse C-Reactive Protein/CRP (Catalog # 1829-CR)

Mouse C-Reactive Protein/CRP Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse C‑Reactive Protein/CRP Antibody (Catalog # MAB1829)

Standard: Recombinant Mouse C-Reactive Protein/CRP Protein, CF (Catalog # 1829-CR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

C-reactive protein (CRP) is a member of the pentraxin family of plasma proteins that are part of the lectin fold superfamily of calcium-dependent, carbohydrate-binding proteins (1). CRP is named for its ability to bind to the C-polysaccharide of Strep. Pneumoniae. CRP is characterized by cyclic pentameric structure that contains five identical protomers/subunits, each exhibiting a lectin fold composed of two antiparallel beta -sheets with a fattened jellyroll topology. The mouse CRP precursor is 225 amino acids (aa) in length and contains a signal peptide of 19 aa with a mature polypeptide of 206 aa (2, 3). There is one intrachain disulfide bond and no N-linked glycosylation site(s). Although rat CRP is glycosylated at an N-linked site, human, mouse and rabbit CRP all appear to be non-glycosylated (1, 4, 5). In mouse, the protomers are assembled non-covalently to form the pentameter; in rat, two of the five protomers are covalently linked (6). Mature mouse CRP shares 74%, 71%, 79%, and 68% aa sequence identity with rat, human, hamster and guinea pig CRP, respectively. In human, CRP is induced in hepatocytes principally by IL-6 (1). In mouse, IL-6 has very little effect. Mouse CRP induction is due principally to IL-1 (1, 7), with another pentraxin, SAP, being IL-6 inducible (7). CRP exhibits calcium-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP will also bind to the cell membrane of injured necrotic and apoptotic cells. In this context, CRP acts as an opsonin, binding to Fc gamma RI and II, and serves as an anti-inflammatory agent (8).

References
  1. Hirschfield, G.M. and M.B. Pepys (2003) Q.J. Med 96:793.
  2. Whitehead, A.S. et al. (1990) Biochem. J. 266:283.
  3. Ohnishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 156:814.
  4. Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
  5. Black, S. et al. (2004) J. Biol. Chem. 279:48487.
  6. Rassouli, M. et al. (1992) J. Biol. Chem. 267:2947.
  7. Ku, N-O. and R.F. Mortensen (1993) Cytokine 5:319.
  8. Mold, C. et al. (2002) J. Immunol. 169:7019.
Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Product Datasheets

Citation for Mouse C-Reactive Protein/CRP Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice.
    Authors: Teupser D, Weber O, Rao TN, Sass K, Thiery J, Fehling HJ
    J. Biol. Chem., 2011;286(8):6272-9.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot

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