Mouse Cathepsin B Antibody AF965: R&D Systems

Mouse Cathepsin B Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Cathepsin B is observed and less than 5% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin C, and rmCathepsin D is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
    His18-Phe339
    Accession # P10605
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Cathepsin B (Catalog # 965-CY)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Recombinant Mouse Cathepsin B (0.1 µg/mL, Catalog # 965-CY) cleavage of the fluorogenic peptide substrate Z-LR-AMC (10 µM, Catalog # ES008). The Neutralization Dose (ND50) is typically 0.33 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Cathepsin B in Mouse Thymus. Cathepsin B was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Goat Anti-Mouse Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF965) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin B
Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2‑4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution in human and animal tumors, Cathepsin B may also have a role in invasion and metastasis (5). In addition to the lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (5).
  • References:
    1. Mort, J.S. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.) p. 1079, Academic Press, San Diego.
    2. Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
    3. Jutras, I. and T.L. Reudelhuber (1998) FEBS Lett. 443:48.
    4. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
    5. Berquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.
  • Entrez Gene IDs:
    1508 (Human); 13030 (Mouse)
  • Alternate Names:
    APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models
    Authors: K Takahashi, H Mashima, K Miura, D Maeda, A Goto, T Goto, GH Sun-Wada, Y Wada, H Ohnishi
    Sci Rep, 2017;7(1):2817.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  3. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
    Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
    Mol Cancer, 2015;14(0):39.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Inflammasome activation by altered proteostasis.
    Authors: Shin, Jin Na, Fattah, Elmoataz, Bhattacharya, Abhisek, Ko, Soyoung, Eissa, N Tony
    J Biol Chem, 2013;288(50):35886-95.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation.
    Authors: Lee, Hwa-Youn, Lee, Geum-Hwa, Lee, Mi-Rin, Kim, Hye-Kyun, Kim, Nan-youn, Kim, Seung-Hy, Lee, Yong-Chu, Kim, Hyung-Ry, Chae, Han-Jung
    PLoS ONE, 2013;8(12):e81349.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  6. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Endocytosis provides a major alternative pathway for lysosomal biogenesis in kidney proximal tubular cells.
    Authors: Nielsen R, Courtoy PJ, Jacobsen C, Dom G, Lima WR, Jadot M, Willnow TE, Devuyst O, Christensen EI
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(13):5407-12.
    Species: Mouse
    Sample Type: Serum
    Application: WB
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