Mouse Cathepsin B Antibody

Catalog # Availability Size / Price Qty
AF965
AF965-SP
Mouse Cathepsin B Antibody in Immunohistochemistry
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Product Details
Citations (10)
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Mouse Cathepsin B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cathepsin B is observed and less than 5% cross-reactivity with recombinant mouse Cathepsin H is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
His18-Phe339
Accession # P10605
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Cathepsin B (Catalog # 965-CY)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Recombinant Mouse Cathepsin B (0.1 µg/mL, Catalog # 965-CY) cleavage of the fluorogenic peptide substrate Z-LR-AMC (10 µM, Catalog # ES008). The Neutralization Dose (ND50) is typically 0.33 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Cathepsin B in Mouse Thymus. View Larger

Cathepsin B in Mouse Thymus. Cathepsin B was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Goat Anti-Mouse Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF965) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin B

Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2‑4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution in human and animal tumors, Cathepsin B may also have a role in invasion and metastasis (5). In addition to the lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (5).

References
  1. Mort, J.S. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.) p. 1079, Academic Press, San Diego.
  2. Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
  3. Jutras, I. and T.L. Reudelhuber (1998) FEBS Lett. 443:48.
  4. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  5. Berquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.
Entrez Gene IDs
1508 (Human); 13030 (Mouse)
Alternate Names
APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1

Product Datasheets

Citations for Mouse Cathepsin B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Cysteine-type cathepsins promote the effector phase of acute cutaneous delayed-type hypersensitivity reactions
    Authors: J Schwenck, A Maurer, B Fehrenbach, R Mehling, P Knopf, N Mucha, D Haupt, K Fuchs, CM Griessinge, D Bukala, J Holstein, M Schaller, IG Menendez, K Ghoreschi, L Quintanill, M Gütschow, S Laufer, T Reinheckel, M Röcken, H Kalbacher, BJ Pichler, M Kneilling
    Theranostics, 2019;9(13):3903-3917.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice
    Authors: JK Götzl, AV Colombo, K Fellerer, A Reifschnei, G Werner, S Tahirovic, C Haass, A Capell
    Mol Neurodegener, 2018;13(1):48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons
    Authors: XT Cheng, YX Xie, B Zhou, N Huang, T Farfel-Bec, ZH Sheng
    J. Cell Biol., 2018;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models
    Authors: K Takahashi, H Mashima, K Miura, D Maeda, A Goto, T Goto, GH Sun-Wada, Y Wada, H Ohnishi
    Sci Rep, 2017;7(1):2817.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
    Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
    Mol Cancer, 2015;14(0):39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Inflammasome activation by altered proteostasis.
    Authors: Shin, Jin Na, Fattah, Elmoataz, Bhattacharya, Abhisek, Ko, Soyoung, Eissa, N Tony
    J Biol Chem, 2013;288(50):35886-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation.
    Authors: Lee, Hwa-Youn, Lee, Geum-Hwa, Lee, Mi-Rin, Kim, Hye-Kyun, Kim, Nan-youn, Kim, Seung-Hy, Lee, Yong-Chu, Kim, Hyung-Ry, Chae, Han-Jung
    PLoS ONE, 2013;8(12):e81349.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  9. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Endocytosis provides a major alternative pathway for lysosomal biogenesis in kidney proximal tubular cells.
    Authors: Nielsen R, Courtoy PJ, Jacobsen C, Dom G, Lima WR, Jadot M, Willnow TE, Devuyst O, Christensen EI
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(13):5407-12.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot

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