Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 192204
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin F
Ser20-Asn462
Accession # Q9WUT4
Ser20-Asn462
Accession # Q9WUT4
Specificity
Detects mouse Cathepsin F in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant mouse Cathepsin B, C, L, or S is observed and approximately 5% and 25% cross-reactivity with recombinant human Cathepsin F under non-reducing conditions and reducing conditions, respectively, is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Mouse Cathepsin F Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin F, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin F, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Mouse Cathepsin F
Sample: Recombinant Mouse Cathepsin F
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cathepsin F
Additional Cathepsin F Products
Product Documents for Mouse Cathepsin F Antibody
Product Specific Notices for Mouse Cathepsin F Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars