Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/ MCP‑5 Antibody. |
Recombinant Mouse CCL12/MCP‑5 (Catalog # 428‑P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB4281). The ND50 is typically 0.5-2.5 µg/mL.
CCL12, also called MCP-5, is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. Mouse CCL12 encodes a 104 amino acid (aa) precursor protein with a 22 aa signal sequence that is cleaved to generate a 82 aa mature protein. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. CCL12 has been found to be a functional ligand for CCR2.
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