Mouse CCL12/MCP-5 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL12/MCP‑5 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 147801
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL12/MCP‑5
    Gly23-Gly104
    Accession # Q62401
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL12/MCP‑5 and Neutral­ization by Mouse CCL12/ MCP‑5 Antibody.

Recombinant Mouse CCL12/MCP‑5 (Catalog # 428‑P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB4281). The ND50 is typically 0.5-2.5 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL12/MCP-5

CCL12, also called MCP-5, is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. Mouse CCL12 encodes a 104 amino acid (aa) precursor protein with a 22 aa signal sequence that is cleaved to generate a 82 aa mature protein. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. CCL12 has been found to be a functional ligand for CCR2.

  • Entrez Gene IDs:
    20293 (Mouse)
  • Alternate Names:
    C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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APC-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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