Mouse CCL22/MDC Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL22/MDC
    Gly25-Ser92
    Accession # Q9QZU2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL22/MDC (Catalog # 439-MD)
  • Neutralization
    Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse CCL22/MDC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. Recombinant Mouse CCL22/MDC (Catalog # 439‑MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND50 is typically 0.3-1.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL22/MDC

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

  • References:
    1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
    2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
    3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
    4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
  • Entrez Gene IDs:
    6367 (Human); 20299 (Mouse)
  • Alternate Names:
    A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
    Authors: Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G
    J Bone Miner Res, 2015;30(3):412-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
    Authors: Loebbermann, Jens, Durant, Lydia, Thornton, Hannah, Johansson, Cecilia, Openshaw, Peter J
    Proc Natl Acad Sci U S A, 2013;110(8):2987-92.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
    Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
    J. Clin. Invest., 2011;121(0):3024.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008;205(3):699-710.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Functional Assay
  7. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
    Authors: Carpenter KJ, Hogaboam CM
    Infect. Immun., 2005;73(11):7198-207.
    Species: Mouse
    Sample Type:
    Application: Neut
Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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