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Mouse CCL22/MDC Antibody

Catalog #: AF439
12 Citations Datasheet / COA / SDS
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AF439
AF439-SP

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Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody.
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL22/MDC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # Q9QZU2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL22/MDC (Catalog # 439-MD)
Neutralization
Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse CCL22/MDC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. View Larger

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. Recombinant Mouse CCL22/MDC (Catalog # 439-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND50 is typically 0.3-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL22/MDC

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

References
  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
Entrez Gene IDs
6367 (Human); 20299 (Mouse)
Alternate Names
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

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Citations for Mouse CCL22/MDC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Cardiomyocyte Expression of ZO-1 Is Essential for Normal Atrioventricular Conduction but Does Not Alter Ventricular Function
    Authors: Jianlin Zhang, Kevin P. Vincent, Angela K. Peter, Matthew Klos, Hongqiang Cheng, Selina Manying Huang et al.
    Circulation Research
  2. CCR4 and CCR7 differentially regulate thymocyte localization with distinct outcomes for central tolerance
    Authors: Yu Li, Pablo Guaman Tipan, Hilary J Selden, Jayashree Srinivasan, Laura P Hale, Lauren I R Ehrlich
    eLife
  3. PGE2-EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment
    Authors: D Thumkeo, S Punyawatth, S Prasongtan, R Matsuura, K Arima, H Nie, R Yamamoto, N Aoyama, H Hamaguchi, S Sugahara, S Takeda, V Charoensaw, A Tanaka, S Sakaguchi, S Narumiya
    Cell Reports, 2022-06-07;39(10):110914.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Prolactin enhances T regulatory cell promotion of breast cancer through the long form prolactin receptor
    Authors: KE Chen, M Ghosh, L Rivera, S Lin, A Kumar, S Swaminatha, MY Lorenson, AM Walker
    Translational Oncology, 2021-08-07;14(11):101195.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge
    Authors: S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang
    Nat Commun, 2018-09-24;9(1):3879.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
    Authors: Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G
    J Bone Miner Res, 2015-03-01;30(3):412-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
    Authors: Loebbermann, Jens, Durant, Lydia, Thornton, Hannah, Johansson, Cecilia, Openshaw, Peter J
    Proc Natl Acad Sci U S A, 2013-02-04;110(8):2987-92.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
    Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
    J. Clin. Invest., 2011-08-01;121(0):3024.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008-03-03;205(3):699-710.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Functional Assay
  12. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
    Authors: Carpenter KJ, Hogaboam CM
    Infect. Immun., 2005-11-01;73(11):7198-207.
    Species: Mouse
    Sample Types:
    Applications: Neutralization

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