Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 89827
View all CCL25/TECK Primary Antibodies »

Product Specifications

Specificity

Detects mouse CCL25/TECK in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, and recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CCL25/TECK Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CCL25/TECK

CCL25 (thymus-expressed chemokine), also known as TECK (thymus-expressed chemokine), is a CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein.  Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. CCL25 functions through interactions with CCR9 and is chemotactic for activated macrophages, dendritic cells and thymocytes (1).

References

  1. Vicari, A.P. et al. (1997) Immunity 7:291.

Alternate Names

TECK

Entrez Gene IDs

6370 (Human); 20300 (Mouse)

Gene Symbol

CCL25

UniProt

O35903.1

Additional CCL25/TECK Products

Product Documents for Mouse CCL25/TECK Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCL25/TECK Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Chemokines and Receptors

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Protocols

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