Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL28 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2

Specificity

Detects mouse CCL28 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human CCL28 and recombinant mouse CTACK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CCL28 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL28 (Catalog # 533-VI)

Mouse CCL28 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CCL28 Antibody (Catalog # MAB5331)
  • Standard: Recombinant Mouse CCL28 Protein (Catalog # 533-VI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

References

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Alternate Names

CCL28, MEC, VIC

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Gene Symbol

CCL28

UniProt

Q9JIL2

Additional CCL28 Products

Product Documents for Mouse CCL28 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CCL28 Biotinylated Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse CCL28 Biotinylated Antibody

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