Mouse CCL9/10/MIP-1 gamma Antibody

Catalog # Availability Size / Price Qty
MAB463-SP
MAB463-500
MAB463-100
Chemotaxis Induced by CCL9/10/MIP‑1 gamma  and Neutral-ization by Mouse CCL9/10/ MIP‑1 gamma  Antibody.
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Citations (8)
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Mouse CCL9/10/MIP-1 gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL9/10/MIP‑1 gamma in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human CCL3, 4, 15, 19, 20, recombinant mouse CCL3, 4, 19, or recombinant rat CCL20 is observed.
Source
Monoclonal Rat IgG1 Clone # 62105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma
Gln22-Gln122
Accession # P51670
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG) under non-reducing conditions only

Mouse CCL9/10/MIP-1 gamma Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody (Catalog # BAF463)

Standard: Recombinant Mouse CCL9/10/MIP-1 gamma Protein (Catalog # 463-MG)

Neutralization
Measured by its ability to neutralize CCL9/10/MIP‑1 gamma -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL9/10/MIP‑1 gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL9/10/MIP‑1 gamma  and Neutral-ization by Mouse CCL9/10/ MIP‑1 gamma  Antibody. View Larger

Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutral-ization by Mouse CCL9/10/ MIP‑1 gamma Antibody. Recombinant Mouse CCL9/10/ MIP-1 gamma (Catalog # 463-MG) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP-1 gamma (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL9/10/MIP-1 gamma Monoclonal Antibody (Catalog # MAB463). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full‑length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

References
  1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
  3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
  4. Haelens, A. et al. (1996) Immunobiology 195:499.
  5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
  6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
  7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.
Entrez Gene IDs
20308 (Mouse)
Alternate Names
CCF18; CCL9/10; MIP1 gamma; MIP-1 gamma

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Citations for Mouse CCL9/10/MIP-1 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Chemokine CCL9 Is Upregulated Early in Chronic Kidney Disease and Counteracts Kidney Inflammation and Fibrosis
    Authors: C Hemmers, C Schulte, J Wollenhaup, DWL Wong, E Harlacher, S Orth-Alamp, BM Klinkhamme, SH Schirmer, M Böhm, N Marx, T Speer, P Boor, J Jankowski, H Noels
    Biomedicines, 2022-02-10;10(2):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Cytotoxic T cells swarm by homotypic chemokine signalling
    Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
    eLife
  3. Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury
    Authors: L Li, L Ni, RF Heary, S Elkabes
    J Neuroinflammation, 2020-02-25;17(1):73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Novel chemokine-like activities of histones in tumor metastasis
    Authors: R Chen, Y Xie, X Zhong, Y Fu, Y Huang, Y Zhen, P Pan, H Wang, DL Bartlett, TR Billiar, MT Lotze, HJ Zeh, XG Fan, D Tang, R Kang
    Oncotarget, 2016-09-20;7(38):61728-61740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Loss of muscleblind-like 1 promotes invasive mesenchyme formation in endocardial cushions by stimulating autocrine TGF beta 3
    Authors: Kathryn E LeMasters, Yotam Blech-Hermoni, Samantha J Stillwagon, Natalie A Vajda, Andrea N Ladd
    BMC Developmental Biology
  6. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  7. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004-08-01;173(3):2084-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004-06-01;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development, ELISpot Development

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