Mouse CCL9/10/MIP-1 gamma Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL9/10/MIP‑1 gamma in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human CCL3, 4, 15, 19, 20, recombinant mouse CCL3, 4, 19, or recombinant rat CCL20 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 62105
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma
    Gln22-Gln122
    Accession # P51670
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG) under non-reducing conditions only
    • Mouse CCL9/10/MIP-1 gamma Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CCL9/10/MIP‑1 gamma Antibody (Catalog # MAB463)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody (Catalog # BAF463)
  • ELISA Standard
     
    Recombinant Mouse CCL9/10/MIP-1 gamma Protein (Catalog # 463-MG)
  • Neutralization
    Measured by its ability to neutralize CCL9/10/MIP‑1 gamma -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL9/10/MIP‑1 gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutral­ization by Mouse CCL9/10/
MIP‑1 gamma Antibody.
Recombinant Mouse CCL9/10/
MIP‑1 gamma (Catalog # 463-MG) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP‑1 gamma (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL9/10/MIP‑1 gamma Monoclonal Antibody (Catalog # MAB463). The ND50 is typically 1-4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL9/10/MIP-1 gamma
Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full‑length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).
  • References:
    1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
    2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
    3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
    4. Haelens, A. et al. (1996) Immunobiology 195:499.
    5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
    6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
    7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.
  • Entrez Gene IDs:
    20308 (Mouse)
  • Alternate Names:
    CCF18; CCL9/10; MIP1 gamma; MIP-1 gamma
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Novel chemokine-like activities of histones in tumor metastasis
    Authors: R Chen, Y Xie, X Zhong, Y Fu, Y Huang, Y Zhen, P Pan, H Wang, DL Bartlett, TR Billiar, MT Lotze, HJ Zeh, XG Fan, D Tang, R Kang
    Oncotarget, 2016;7(38):61728-61740.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  3. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004;173(3):2084-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  5. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004;173(3):2084-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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