Mouse CCR1 APC‑conjugated Antibody

R&D Systems | Catalog # FAB5986A

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 643854
View all CCR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCR1
Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
Accession # P51675

Specificity

Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR1 APC‑conjugated Antibody

Detection of CCR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5986A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR1 antibody in L1.2 Mouse Cell Line Transfected with Human CCR1 and eGFP antibody by Flow Cytometry.

Detection of CCR1 in L1.2 Mouse Cell Line Transfected with Human CCR1 and eGFP by Flow Cytometry.

L1.2 mouse pro-B cell line transfected with mouse CCR1 and eGFP was stained with Rat Anti-Mouse CCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5986A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line and L1.2 mouse pro-B cell line transfected with mouse CCR1 and eGFP

Reviewed Applications

Read 2 reviews rated 4 using FAB5986A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR1

CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 

Alternate Names

CCR1, CD191

Entrez Gene IDs

1230 (Human); 12768 (Mouse); 57301 (Rat)

Gene Symbol

CCR1

UniProt

P51675

Additional CCR1 Products

Product Documents for Mouse CCR1 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR1 APC‑conjugated Antibody

For research use only

Citations for Mouse CCR1 APC‑conjugated Antibody

Customer Reviews for Mouse CCR1 APC‑conjugated Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
50%
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1 Stars
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Customer Images

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  • Mouse CCR1 APC-conjugated Antibody
    Name: Kihong Lim
    Application: Flow Cytometry
    Sample Tested: activated mouse CD8 T cell
    Species: Mouse
    Verified Customer | Posted 09/29/2016
    CCR1 expression on mouse CD8 T cell activated in vitro or in virus-infected lung
    Mouse CCR1 APC‑conjugated Antibody FAB5986A
  • Mouse CCR1 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 08/24/2016

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