Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5986A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR1 in L1.2 Mouse Cell Line Transfected with Human CCR1 and eGFP by Flow Cytometry.
L1.2 mouse pro-B cell line transfected with mouse CCR1 and eGFP was stained with Rat Anti-Mouse CCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5986A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively.
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We have 2 review tested in 1 species: Mouse.
We have 2 review tested in 1 application: Flow Cytometry.