Mouse CCR1 APC-conjugated Antibody

(2 Reviews)
  • Species Reactivity
  • Specificity
    Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 643854
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCR1
    Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
    Accession # P51675
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5986A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR1 in L1.2 Mouse Cell Line Transfected with Human CCR1 and eGFP by Flow Cytometry. L1.2 mouse pro-B cell line transfected with mouse CCR1 and eGFP was stained with Rat Anti-Mouse CCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5986A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR1
CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 
  • Entrez Gene IDs:
    1230 (Human); 12768 (Mouse); 57301 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse CCR1 APC-conjugated Antibody FAB5986A
 Flow Mouse Anonymous 09/29/2016
Image Details
Flow Cytometry Mouse CCR1 APC-conjugated Antibody FAB5986A
Flow Cytometry: Mouse CCR1 APC-conjugated Antibody [FAB5986A]


ApplicationFlow Cytometry
Sample TestedActivated mouse CD8 T cell

Other Experimental Details

Other Experimental DetailsCCR1 expression on mouse CD8 T cell activated in vitro or in virus-infected lung
 Flow Mouse Anonymous 08/24/2016


ApplicationFlow Cytometry
Sample TestedTumor cell lyastes

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