Mouse CCR1 Antibody

Catalog # Availability Size / Price Qty
MAB5986
MAB5986-SP
Detection of Mouse CCR1 by Western Blot.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse CCR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.
Source
Monoclonal Rat IgG2B Clone # 643854
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCR1
Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
Accession # P51675
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse CCR1 by Western Blot. View Larger

Detection of Mouse CCR1 by Western Blot. Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR1

CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a
50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 

Entrez Gene IDs
1230 (Human); 12768 (Mouse); 57301 (Rat)
Alternate Names
C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R

Product Datasheets

You must select a language.

x

Citations for Mouse CCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype
    Authors: C Xie, F Ye, N Zhang, Y Huang, Y Pan, X Xie
    Journal of Cellular and Molecular Medicine, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
    Authors: Lewis, Nuruddee, Muthukumarana, Akalushi, Fogal, Steven E, Corradini, Laura, Stefanopoulos, Dimitria, Adusumalli, Prathima, Pelletier, Josephin, Panzenbeck, Mark, Berg, Karen, Canfield, Melissa, Cook, Brian N, Razavi, Hossein, Kuzmich, Daniel, Anderson, Shawn, Allard, Devan, Harrison, Paul, Grimaldi, Christin, Souza, Donald, Harcken, Christia, Fryer, Ryan M, Modis, Louise K, Brown, Maryanne
    PLoS ONE, 2014;9(8):e105883.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MIP-1alpha/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia.
    Authors: Baba T, Naka K, Morishita S, Komatsu N, Hirao A, Mukaida N
    J Exp Med, 2013;210(12):2661-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse CCR1 Antibody

There are currently no reviews for this product. Be the first to review Mouse CCR1 Antibody and earn rewards!

Have you used Mouse CCR1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review