Mouse CCR3 Fluorescein-conjugated Antibody

(20 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
  • Source
    Monoclonal Rat IgG2A Clone # 83101
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with mouse CCR3
    Met1-Phe359
    Accession # P51678
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse  splenocytes were stained with Rat Anti-Mouse CCR3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB729F) and Rat Anti-Mouse Gr‑1/Ly‑6G APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A). Quadrant markers were set based on control antibody staining (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR3
CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.
  • Entrez Gene IDs:
    1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
  • Alternate Names:
    b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production
    Authors: P Rozenberg, H Reichman, I Zab-Bar, M Itan, M Pasmanik-C, C Bouffi, U Qimron, I Bachelet, PC Fulkerson, ME Rothenberg, A Munitz
    Sci Rep, 2017;7(1):5922.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Frontline Science: Eosinophil-deficient MBP-1 and EPX double-knockout mice link pulmonary remodeling and airways dysfunction with type 2 inflammation
    Authors: SI Ochkur, AD Doyle, EA Jacobsen, WE LeSuer, W Li, CA Protheroe, KR Zellner, D Colbert, HH Shen, CG Irvin, JJ Lee, NA Lee
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge.
    Authors: Takeda K, Shiraishi Y, Ashino S, Han J, Jia Y, Wang M, Lee N, Lee J, Gelfand E
    J Allergy Clin Immunol, 2015;135(2):451-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Generation of eosinophils from cryopreserved murine bone marrow cells.
    Authors: Schollaert, Kaila L, Stephens, Michael, Gray, Jerilyn, Fulkerson, Patricia
    PLoS ONE, 2014;9(12):e116141.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
    Authors: Doyle, Alfred D, Jacobsen, Elizabet, Ochkur, Sergei I, Willetts, Lian, Shim, Kelly, Neely, Joseph, Kloeber, Jake, Lesuer, Will E, Pero, Ralph S, Lacy, Paige, Moqbel, Redwan, Lee, Nancy A, Lee, James J
    J Leukoc Biol, 2013;94(1):17-24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Targeted ablation of miR-21 decreases murine eosinophil progenitor cell growth.
    Authors: Lu, Thomas X, Lim, Eun-Jin, Itskovich, Svetlana, Besse, John A, Plassard, Andrew J, Mingler, Melissa, Rothenberg, Joelle A, Fulkerson, Patricia, Aronow, Bruce J, Rothenberg, Marc E
    PLoS ONE, 2013;8(3):e59397.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Flow
  8. Eosinophil-expressed galectin-3 regulates cell trafficking and migration.
    Authors: Ge X, Ha S, Liu F, Rao S, Sriramarao P
    Front Pharmacol, 2013;4(0):37.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
    Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
    PLoS ONE, 2011;6(5):e19957.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
    Authors: Yamada T, Tani Y, Nakanishi H
    FASEB J., 2011;25(2):561-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection.
    Authors: Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M
    J. Immunol., 2011;187(5):2656-65.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation.
    Authors: Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P
    J. Biol. Chem., 2011;286(44):38231-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. SIRP{alpha}/CD172a Regulates Eosinophil Homeostasis.
    Authors: Verjan Garcia N, Umemoto E, Saito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M
    J. Immunol., 2011;187(5):2268-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. CD28 and ICOS play complementary non-overlapping roles in the development of Th2 immunity in vivo.
    Authors: Shilling RA, Clay BS, Tesciuba AG, Berry EL, Lu T, Moore TV, Bandukwala HS, Tong J, Weinstock JV, Flavell RA, Horan T, Yoshinaga SK, Welcher AA, Cannon JL, Sperling AI
    Cell. Immunol., 2009;259(2):177-84.
    Species: Mouse
    Sample Type: BALF
    Application: Flow
  15. CD43 regulates Th2 differentiation and inflammation.
    Authors: Cannon JL, Collins A, Mody PD, Balachandran D, Henriksen KJ, Smith CE, Tong J, Clay BS, Miller SD, Sperling AI
    J. Immunol., 2008;180(11):7385-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  16. Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation.
    Authors: Gupta S, Fuchs B, Schulz-Maronde S, Heitland A, Escher SE, Mack M, Tillmann HC, Braun A, Forssmann WG, Elsner J, Forssmann U
    J. Leukoc. Biol., 2007;83(3):765-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
    Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O'Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
    J. Immunol., 2007;178(12):7879-89.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  18. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells.
    Authors: Radinger M, Bossios A, Alm AS, Jeurink P, Lu Y, Malmhall C, Sjostrand M, Lotvall J
    Allergy, 2007;62(12):1410-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
    Authors: Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F
    J. Immunol., 2006;177(1):77-83.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  20. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation.
    Authors: Fulkerson PC, Fischetti CA, McBride ML, Hassman LM, Hogan SP, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(44):16418-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 20 Citations
Isotype Controls
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Rat IgG2A Fluorescein-conjugated Antibody

Ctrl IC006F 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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