Mouse CCR9 Antibody

Catalog # Availability Size / Price Qty
MAB2160-100
MAB2160-SP
Detection  of CCR9 in Mouse Thymocytes by Flow Cytometry.
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Mouse CCR9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 242503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR9
Met1-Leu369
Accession # Q9WUT7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CCR9 in Mouse Thymocytes by Flow Cytometry. View Larger

Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Monoclonal Antibody (Catalog # MAB2160, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs
10803 (Human); 12769 (Mouse); 282832 (Rat)
Alternate Names
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

Citations for Mouse CCR9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Gut memories do not fade: epigenetic regulation of lasting gut homing receptor expression in CD4(+) memory T cells
    Authors: BA Szilagyi, J Triebus, C Kressler, M de Almeida, S Tierling, P Durek, M Mardahl, A Szilagyi, S Floess, J Huehn, U Syrbe, J Walter, JK Polansky, A Hamann
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
    Sci Rep, 2016;6(0):37914.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells.
    Authors: Bakdash G, Vogelpoel L, Van Capel T, Kapsenberg M, de Jong E
    Mucosal Immunol, 2015;8(2):265-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  4. TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice.
    Authors: Heidegger S, Kirchner S, Stephan N, Bohn B, Suhartha N, Hotz C, Anz D, Sandholzer N, Stecher B, Russmann H, Endres S, Bourquin C
    J Immunol, 2013;190(10):5313-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  5. E2A proteins promote development of lymphoid-primed multipotent progenitors.
    Authors: Dias S, Mansson R, Gurbuxani S, Sigvardsson M, Kee BL
    Immunity, 2008;29(2):217-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  6. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  7. Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
    Authors: Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
    J. Leukoc. Biol., 2007;81(1):176-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  8. Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization.
    Authors: Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y
    Blood, 2006;108(8):2531-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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Mouse CCR9 Antibody
By Anonymous on 08/04/2016
Application: Flow Sample Tested: T cells Species: Mouse

CD4 T cells from spleen and intestines were stained.