Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # PK136
View all CD161/NK1.1 Primary Antibodies »

Product Specifications

Immunogen

NK-1+ cells from mouse spleen and bone marrow
Accession # AAH61168

Specificity

Detects mouse CD161/NK1.1 in Flow Cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Mouse CD161/NK1.1 Biotinylated Antibody

Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry.

Mouse C57BL/6 splenocytes were stained with (A) Mouse Anti-Mouse CD161/NK1.1 biotinylated Monoclonal Antibody (Catalog # FAB76141B) or (B) Mouse IgG2A Isotype Control (IC003B) followed by Allophycocyanin-conjugated Streptavidin (F0050) and Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Mouse CD161/NK1.1 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: C57BL/6 splenocytes

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Entrez Gene IDs

17059 (Mouse)

Gene Symbol

KLRB1C

UniProt

AAH61168

Additional CD161/NK1.1 Products

Product Documents for Mouse CD161/NK1.1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD161/NK1.1 Biotinylated Antibody

For research use only

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