Mouse CD24 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD24a in direct ELISAs.
  • Source
    Recombinant Monoclonal Rat IgG2A Clone # 910019
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Peptide sequence spanning a portion of the extracellular region
    Accession # P24807
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD24 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD24 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB8547G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD24

CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.

  • Entrez Gene IDs:
    100133941 (Human); 12484 (Mouse)
  • Alternate Names:
    CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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