|Detection of CD24 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD24 Monoclonal Antibody (Catalog # MAB8547) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).|
|CD24 in Mouse Stomach. CD24 was detected in immersion fixed paraffin-embedded sections of mouse stomach using Rat Anti-Mouse CD24 Monoclonal Antibody (Catalog # MAB8547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to parietal cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided