Mouse CD25/IL-2 R alpha PerCP-conjugated Antibody

Discontinued Product

FAB2438C has been discontinued.
View all CD25/IL-2R alpha products.
Mouse CD25/IL-2 R alpha PerCP-conjugated Antibody in Data
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Mouse CD25/IL-2 R alpha PerCP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) gamma c, recombinant human CD25/IL-2 R alpha, rmIL-2 R beta, or rmIL-15 R is observed.
Source
Monoclonal Rat IgG2A Clone # 280406
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
Glu22-Lys236
Accession # P01590
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD25/IL-2 R alpha PerCP-conjugated Monoclonal Antibody (Catalog # FAB2438C) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for Activation Induced Cell Death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12‑14).

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
  3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
  4. Miller, J. et al. (1985) J. Immunol. 134:4212.
  5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
  6. Hatakeyama, M. et al. (1989) Science 244:551.
  7. Takeshita, T. et al. (1992) Science 257:379.
  8. Wang, X. et al. (2005) Science 310:1159.
  9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
  10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
  11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
  12. Willerford, D.M. et al. (1995) Immunity 3:521.
  13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
  14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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