Mouse CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB9164G-025
Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD25/IL-2 R alpha in flow cytometry.
Source
Monoclonal Rat IgG1 Clone # PC61.5.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
B6.1 mouse cytotoxic T cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD25/IL-2 R alpha antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8214R) and either (A) Rat Anti-Mouse CD25/IL-2 R alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9164G) or (B) Rat IgG1Alexa Fluor 488 Isotype Control (Catalog # IC005G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain. Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor. By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2. Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders. IL-2 R alpha is required for Activation Induced Cell Death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones. IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Mouse CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies
    Authors: T Mondal, GN Shivange, RG Tihagam, E Lyerly, M Battista, D Talwar, R Mosavian, K Urbanek, NS Rashid, JC Harrell, PD Bos, EB Stelow, MS Stack, S Bhatnagar, J Tushir-Sin
    Embo Molecular Medicine, 2021-02-15;13(3):e12716.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Mouse CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review