Mouse CD302/CLEC13A Antibody

Catalog # Availability Size / Price Qty
MAB64241
MAB64241-SP
Detection of Mouse CD302/CLEC13A by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse CD302/CLEC13A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) CD302 is observed. No cross-reactivity with rhCLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, recombinant mouse CLEC4B2, 9A, or 12A is observed.
Source
Monoclonal Rat IgG1 Clone # 662714
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
Met1-Cys165
Accession # Q9DCG2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse CD302/CLEC13A antibody by Western Blot. View Larger

Detection of Mouse CD302/CLEC13A by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse CD302/CLEC13A Monoclonal Antibody (Catalog # MAB64241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD302/CLEC13A

CD302, also known as CLEC13A and DCL-1, is a 35 kDa type I transmembrane glycoprotein in the C-type lectin family. CD302 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. It contains one carbohydrate binding domain in its extracellular region and can mediate antigen uptake. In human Hodgkin’s disease, a fusion protein containing portions of CD302 and the ECD of DEC-205 is expressed on Reed-Sternberg cells.  Within the ECD, mouse CD302 shares 82% and 92% aa sequence identity with human and rat CD302, respectively.

Entrez Gene IDs
9936 (Human); 66205 (Mouse); 295629 (Rat)
Alternate Names
BIMLEC; CD302 antigenC-type lectin domain family 13, member A; CD302 molecule; CD302; CLEC13A; CLEC13AMGC22301; C-type lectin domain family 13 member A; DCL1; DCL-1; DCL1C-type lectin BIMLEC; KIAA0022FLJ43091; Type I transmembrane C-type lectin receptor DCL-1

Product Datasheets

Citation for Mouse CD302/CLEC13A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice
    Authors: RJP Brown, B Tegtmeyer, J Sheldon, T Khera, Anggakusum, D Todt, G Vieyres, R Weller, S Joecks, Y Zhang, S Sake, D Bankwitz, K Welsch, C Ginkel, M Engelmann, G Gerold, E Steinmann, Q Yuan, M Ott, FWR Vondran, T Krey, LJ Ströh, C Miskey, Z Ivics, V Herder, W Baumgärtne, C Lauber, M Seifert, AW Tarr, CP McClure, G Randall, Y Baktash, A Ploss, VLD Thi, E Michailidi, M Saeed, L Verhoye, P Meuleman, N Goedecke, D Wirth, CM Rice, T Pietschman
    Sci Adv, 2020;6(45):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse CD302/CLEC13A Antibody

There are currently no reviews for this product. Be the first to review Mouse CD302/CLEC13A Antibody and earn rewards!

Have you used Mouse CD302/CLEC13A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review