Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) CD302 is observed. No cross-reactivity with rhCLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, recombinant mouse CLEC4B2, 9A, or 12A is observed.
Monoclonal Rat IgG1 Clone # 662714
Protein A or G purified from hybridoma culture supernatant
Detection of Mouse CD302/CLEC13A by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse CD302/CLEC13A Monoclonal Antibody (Catalog # MAB64241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD302, also known as CLEC13A and DCL-1, is a 35 kDa type I transmembrane glycoprotein in the C-type lectin family. CD302 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. It contains one carbohydrate binding domain in its extracellular region and can mediate antigen uptake. In human Hodgkin’s disease, a fusion protein containing portions of CD302 and the ECD of DEC-205 is expressed on Reed-Sternberg cells. Within the ECD, mouse CD302 shares 82% and 92% aa sequence identity with human and rat CD302, respectively.
Entrez Gene IDs:
9936 (Human); 66205 (Mouse); 295629 (Rat)
BIMLEC; CD302 antigenC-type lectin domain family 13, member A; CD302 molecule; CD302; CLEC13A; CLEC13AMGC22301; C-type lectin domain family 13 member A; DCL1; DCL-1; DCL1C-type lectin BIMLEC; KIAA0022FLJ43091; Type I transmembrane C-type lectin receptor DCL-1
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