Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD39 is observed and no cross-reactivity with recombinant mouse CD39L3 or recombinant human CD39L4 is observed.
Monoclonal Rat IgG1 Clone # 495826
Protein A or G purified from hybridoma culture supernatant
Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD39/ENTPD1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4398F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Ectonucleoside Triphosphate Diphosphohydrolase-1 (ENTPD1) is a 65-80 kDa, two-transmembrane glycoprotein that contains an N- and C-terminal cytoplasmic domain (1, 2). ENTPD1 was originally described as CD39, a B lymphocyte cell surface marker and is now known to also be present on the surface of endothelial cells, neutrophils, mast cells, macrophages, pancreatic acinar and duct cells, dendritic cells, sympathetic neuron axon terminals, CD4+ CD39+ CD161+ Th17 precursors, PD-1+ CD39+ "exhausted" CD8+ T cells and FoxP3+ CD25+ CD4+ CD127- CD49d- suppressor plus FoxP3- CD25- CD4+ CD127+ CD49d+ non-suppressor T cells (1-9). ENTPD1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, ENTPD1 plays a role in the regulation of purinergic signaling. Extracellular ATP released from dead or stressed cell creates a proinflammatory environment. In concert with CD73, the conversion of ATP to adenosine reverses this and creates an antiinflammatory environment (1,2). Over amino acids (aa) 38-478, mouse ENTPD1 shares 90% and 76% aa sequence identity with rat and human ENTPD1, respectively
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