Mouse CD4 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
  • Source
    Monoclonal Rat IgG2B Clone # GK1.5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse CTL clone V4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    1-25 µg/mL
    See below
  • Immunoprecipitation
    Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.
  • Cell depletion
    Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Martin, P. et al. (2000) Blood 96:2511.
  • Costimulation of T Cells
    Bosselut, R. et al. (1999) J. Exp. Med. 190:1517.
  • Inhibition of T Cell Function
    Dialynas, D.P. et al. (1983) J. Immunol. 131:2445; Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Kruman, I.I. et al. (1996) Cell. Immunol. 173:236; Grakoui, A. et al. (1999) Science 285:221.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CD4 in Mouse Intestine. CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Catalog # MAB554) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4
CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
  • Entrez Gene IDs:
    920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
  • Alternate Names:
    CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 3 of 3
Filter your results:

Species
Applications
Sample Type
  1. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012;9(0):193.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
    Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
    J. Virol., 2007;81(3):1241-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
    Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
    J. Invest. Dermatol., 2006;126(8):1792-803.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 22  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Isotype Control

Ctrl MAB0061R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF005 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse CD4 Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Feedback

Customer Information