Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Monoclonal Rat IgG2B Clone # GK1.5
Protein A or G purified from hybridoma culture supernatant
Mouse CTL clone V4
<0.10 EU per 1 μg of the antibody by the LAL method.
2.5 µg/106 cells
Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.
Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Martin, P. et al. (2000) Blood 96:2511.
Costimulation of T Cells
Bosselut, R. et al. (1999) J. Exp. Med. 190:1517.
Inhibition of T Cell Function
Dialynas, D.P. et al. (1983) J. Immunol. 131:2445; Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Kruman, I.I. et al. (1996) Cell. Immunol. 173:236; Grakoui, A. et al. (1999) Science 285:221.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
is a type I membrane glycoprotein belonging to the immunoglobulin
superfamily. It is expressed predominantly on thymocytes and a subset of
mature T lymphocytes. CD4 functions in collaboration with the T cell
receptor in the recognition of peptide antigens that are presented by
class II major histocompatibility complexes. CD4 also has been shown to
be a coreceptor of HIV entry and specifically binds gp120, the external
envelope glycoprotein of HIV.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.