Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Cell depletion, Costimulation of T Cells, Inhibition of T Cell Function, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Cell Selection, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # GK1.5
View all CD4 Primary Antibodies »

Product Specifications

Immunogen

Mouse CTL clone V4

Specificity

Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CD4 Antibody

CD4 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

CD4 in Mouse Intestine.

CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Catalog # MAB554) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of CD4 by Flow Cytometry

Detection of CD4 by Flow Cytometry

T cells and Con A-induced hepatitis. Isotype, CD4 and/or CD8 mAbs (100 µg per mouse) was administrated i.p. to Balb/c mice (n = 8 per group) 24h before Con A injection (20 mg kg–1). The effect of anti-CD4 orCD-8 antibodies administration on depleting these cells in the liver of mice prior injecting Con A was determined by FACS. Representative results were shown in (A). Sera were collected 24 h after Con A injection. Serum ALT (B) and AST (C) levels were measured. The results were presented as the mean ± SD of three separate experiments. ***, p<0.001 vs control. (D) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E) staining. Representative liver sections were shown for each group, original magnification: ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23118999), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD4 by Immunohistochemistry

Detection of CD4 by Immunohistochemistry

T cells and Con A-induced hepatitis. Isotype, CD4 and/or CD8 mAbs (100 µg per mouse) was administrated i.p. to Balb/c mice (n = 8 per group) 24h before Con A injection (20 mg kg–1). The effect of anti-CD4 orCD-8 antibodies administration on depleting these cells in the liver of mice prior injecting Con A was determined by FACS. Representative results were shown in (A). Sera were collected 24 h after Con A injection. Serum ALT (B) and AST (C) levels were measured. The results were presented as the mean ± SD of three separate experiments. ***, p<0.001 vs control. (D) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E) staining. Representative liver sections were shown for each group, original magnification: ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23118999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD4 Antibody

Application
Recommended Usage

Cell depletion

Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Martin, P. et al. (2000) Blood 96:2511.

Costimulation of T Cells

Bosselut, R. et al. (1999) J. Exp. Med. 190:1517.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed frozen sections of mouse intestine

Immunoprecipitation

Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.

Inhibition of T Cell Function

Dialynas, D.P. et al. (1983) J. Immunol. 131:2445; Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Kruman, I.I. et al. (1996) Cell. Immunol. 173:236; Grakoui, A. et al. (1999) Science 285:221.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB554 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Additional CD4 Products

Product Documents for Mouse CD4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD4 Antibody

For research use only

Citations for Mouse CD4 Antibody

Customer Reviews for Mouse CD4 Antibody (2)

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Customer Images

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  • Mouse CD4 Antibody
    Name: Anonymous
    Application: Protein:Protein interaction
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 08/24/2021
    Binds to murine CD4-his. Immobilize CD4-his on Penta-his sensors, and flow 3 conc of Anti-murine CD4 (100, 50, 25nM). Specific binding of Anti-murine CD4 to murine CD4, no binding to human CD4.
    Mouse CD4 Antibody MAB554
  • Mouse CD4 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: peritoneal granulocytes
    Species: Mouse
    Verified Customer | Posted 05/30/2017

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Protocols

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