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Mouse CD5L MAb (Clone 375020)

Catalog #: MAB2834 Datasheet / COA / SDS

Discontinued Product

MAB2834 has been discontinued.
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Mouse CD5L MAb (Clone 375020) Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD5L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD5L or recombinant mouse CD5 is observed.
Source
Monoclonal Rat IgG2A Clone # 375020
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD5L
Glu22-Val352
Accession # Q9QWK4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD5L (Catalog # 2834-CL) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5L

CD5 Antigen-like (CD5L), also known as Sp alpha, is a secreted protein that is a member of the SCSR (scavenger receptor cysteine rich) superfamily. CD5L/Sp alpha exhibits a high degree of homology with the extracellular regions of CD5 and CD6, exhibiting three SCSR domains. CD5L/Sp alpha is expressed in lymphoid tissues and associates with immunoglobulins of IgM isotype in the plasma/serum.

Long Name
CD5 Antigen-like
Entrez Gene IDs
922 (Human); 11801 (Mouse)
Alternate Names
AIM; API6; apoptosis inhibitor 6; CD5 antigen-like (scavenger receptor cysteine rich family); CD5 antigen-like; CD5 molecule-like; CD5L; CT-2; IgM-associated peptide; PRO229; Spalpha; SP-alpha

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