Mouse CD6 Antibody

Catalog # Availability Size / Price Qty
AF750
AF750-SP
Product Details
Citations (14)
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Mouse CD6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD6 in direct ELISAs and Western blots. In Western blots, approximately 1% cross-reactivity with recombinant human CD6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD6
Leu18-Gly396
Accession # Q91WN5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD6 Fc Chimera (Catalog # 727-CD)
Adhesion Blockade
The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Mouse CD6 Fc Chimera (Catalog # 727-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 50 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. Mouse CD6 is a 626 amino acid (aa) protein with a 24 aa signal sequence, a 372 aa extracellular domain, and a 204 aa cytoplasmic region. The 668 aa human homolog has also been identified. The human and murine proteins share 70% aa identity over their full lengths.

CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti‑CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Additionally, anti‑CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti‑CD6 mAb in conjunction with PMA, anti‑CD2, or anti‑CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins.

References
  1. Gangemi, R. et al. (1989) J. Immunol. 143:2439.
  2. Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
  3. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
  4. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
  5. Whitney, G. et al. (1995) Mol. Immunol. 32:89.
  6. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
  7. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  8. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
  9. Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165.
  10. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
  11. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
  12. Singer, N.G. et al. (1996) Immunology 88:537.
  13. Aruffo, A. et al. (1997) Immunol. Today 18:498.
Entrez Gene IDs
923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)
Alternate Names
CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6

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Citations for Mouse CD6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. High Keratin-7 Expression in Benign Peri-Tumoral Prostatic Glands Is Predictive of Bone Metastasis Onset and Prostate Cancer-Specific Mortality
    Authors: Dariane, C;Clairefond, S;Peant, B;Communal, L;
    Cancers
  2. Increased Intrathecal Activity of Follicular Helper T Cells in Patients With Relapsing-Remitting Multiple Sclerosis
    Authors: Rikke Holm Hansen, Jacob Talbot, Helene Højsgaard Chow, Malene Bredahl Hansen, Sophie Buhelt, Sebastian Herich et al.
    Neurology - Neuroimmunology Neuroinflammation
  3. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration
    Authors: HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute
    Cell Stem Cell, 2018-08-09;0(0):.
  4. Neonatal rhinovirus induces mucous metaplasia and airways hyperresponsiveness through IL-25 and type 2 innate lymphoid cells
    Authors: Jun Young Hong, J. Kelley Bentley, Yutein Chung, Jing Lei, Jessica M. Steenrod, Qiang Chen et al.
    Journal of Allergy and Clinical Immunology
  5. CD166/ALCAM Mediates Proinflammatory Effects of S100B in Delayed Type Hypersensitivity
    Authors: Rüdiger von Bauer, Dimitrios Oikonomou, Alba Sulaj, Sawsan Mohammed, Agnes Hotz-Wagenblatt, Hermann-Josef Gröne et al.
    The Journal of Immunology
  6. Differential Effect of Non-Thermal Plasma RONS on Two Human Leukemic Cell Populations
    Authors: Hager Mohamed, Eric Gebski, Rufranshell Reyes, Samuel Beane, Brian Wigdahl, Fred C. Krebs et al.
    Cancers (Basel)
  7. Insights into Early-Pregnancy Mechanisms: Mast Cells and Chymase CMA1 Shape the Phenotype and Modulate the Functionality of Human Trophoblast Cells, Vascular Smooth-Muscle Cells and Endothelial Cells
    Authors: Ningjuan Zhang, Anne Schumacher, Beate Fink, Mario Bauer, Ana Claudia Zenclussen, Nicole Meyer
    Cells
  8. Heterogeneous Manifestations of Epithelial-Mesenchymal Plasticity of Circulating Tumor Cells in Breast Cancer Patients
    Authors: LA Tashireva, OE Savelieva, ES Grigoryeva, YV Nikitin, EV Denisov, SV Vtorushin, MV Zavyalova, NV Cherdyntse, VM Perelmuter
    International Journal of Molecular Sciences, 2021-03-02;22(5):.
  9. ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-kappaB
    Authors: M Won, KA Park, S Kim, E Ju, Y Ko, H Yoo, H Ro, J Lee, J Oh, EG Lee, SY Kim, SW Nam, HM Shen, MK Yeo, JM Kim, GM Hur
    Cell Death and Differentiation, 2021-11-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  10. Single-cell and spatial transcriptomics reveal aberrant lymphoid developmental programs driving granuloma formation
    Authors: Thomas Krausgruber, Anna Redl, Daniele Barreca, Konstantin Doberer, Daria Romanovskaia, Lina Dobnikar et al.
    Immunity
  11. Structural basis for IL-12 and IL-23 shared receptor usage reveals a gateway for shaping actions on T versus NK cells
    Authors: Caleb R. Glassman, Yamuna Kalyani Mathiharan, Kevin M. Jude, Leon Su, Ouliana Panova, Patrick J. Lupardus et al.
    Cell
  12. Molecular subtype conversion in CTCs as indicator of treatment adequacy associated with metastasis-free survival in breast cancer
    Authors: Grigoryeva ES, A Tashireva L, Alifanov VV et al.
    Scientific reports
  13. Polystyrene microspheres enable 10‐color compensation for immunophenotyping of primary human leukocytes
    Authors: Tiara Byrd, Karen D. Carr, John C. Norman, Leslie Huye, Meenakshi Hegde, Nabil Ahmed
    Cytometry Part A
  14. Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques
    Authors: Aid M, Busman-Sahay K, Vidal SJ et al.
    Cell

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