Mouse CD96 APC-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD96 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD96v2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 630612
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD96
    Val22-Met536
    Accession # Q3U0X8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD96 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD96 APC‑conjugated Monoclonal Antibody (Catalog # FAB5690A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily.  It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma δ T cells.  Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion.  Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein.  It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region.  The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355).  Unlike human, there is no splice variant in the second V-type domain.  There is, however, a potential isoform that shows a single Cys substitution for aa 437-602.  Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

  • Entrez Gene IDs:
    10225 (Human); 84544 (Mouse); 498079 (Rat)
  • Alternate Names:
    CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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