Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily. It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma delta T cells. Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355). Unlike human, there is no splice variant in the second V-type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437-602. Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Val22-Met536
Accession # Q3U0X8
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse CD96 Antibody
Detection of CD96 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were labeled with Mouse CD96 Monoclonal Antibody (Catalog # MAB5690) followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113) and Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
Applications for Mouse CD96 Antibody
CyTOF-ready
Flow Cytometry
Sample: Mouse splenocytes
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD96
Alternate Names
Gene Symbol
UniProt
Additional CD96 Products
Product Documents for Mouse CD96 Antibody
Product Specific Notices for Mouse CD96 Antibody
For research use only
Customer Reviews for Mouse CD96 Antibody
There are currently no reviews for this product. Be the first to review Mouse CD96 Antibody and earn rewards!
Have you used Mouse CD96 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
