Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 469114
View all CDCP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
Arg25-Ala667
Accession # Q5U462

Specificity

Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CDCP1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse CDCP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CDCP1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDCP1

CUB domain containing protein 1 (CDCP1), also known as SIMA135, is a 140 kDa type I transmembrane glycoprotein with three CUB protein‑protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

Q5U462

Additional CDCP1 Products

Product Documents for Mouse CDCP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CDCP1 Antibody

For research use only

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FAQs

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