Detects mouse Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CHRDL2, recombinant mouse (rm) CHRDL1, or rmChordin is observed.
Monoclonal Rat IgG2A Clone # 328905
Protein A or G purified from hybridoma culture supernatant
Chordin-like 2/CHRDL2 in Embryonic Mouse Cartilage. Chordin-like 2/ CHRDL2 was detected in immersion fixed frozen sections of embryonic mouse cartilage (15 d.p.c.) using 25 µg/mL Rat Anti-Mouse Chordin-like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB2520) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chordin-like 2/CHRDL2
Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is a secreted glycoprotein that functions as a BMP antagonist. It is expressed in chondrocytes in developing joint cartilage, osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG. It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses. CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation.
Entrez Gene IDs:
25884 (Human); 69121 (Mouse)
BNF-1; Chordin like 2; chordin-like 2; chordin-like protein 2; CHRDL2
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