|CLEC9a in Mouse Spleen. CLEC9a was detected in perfusion fixed frozen sections of mouse spleen using Sheep Anti-Mouse CLEC9a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6776) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.|
CLEC9a (C-type lectin domain family member A; also DNGR-1) is a 50-52 kDa member of the group V C-type lectin domain containing family of receptors. In mouse, it is an activation receptor expressed on both CD8 alpha + CD24+ and plasmacytoid dendritic cells (DC). It assists in endocytosis but not phagocytosis, and appears to induce cytotoxic antitumor T cells by participating in class I MHC antigen presentation. CD8 alpha + DCs are believed to be particularly important for the disposal of dead cell material. Mouse CLEC9a is a 264 amino acid (aa) type II transmembrane glycoprotein. It contains a 35 aa cytoplasmic segment (aa 1-35) and a 208 aa extracellular region (aa 57-264) that possesses one C-type lectin domain (aa 144-256). CLEC9a forms disulfide-linked homodimers on the cell surface. There are multiple splice variants. The CLEC9a SwissProt entry (Q8BRU4) is 238 aa in length and shows a deletion of aa 106-131. Other isoforms contain either a 43 or a 67 aa substitution for aa 106-264, a Val substitution for 31-58, and a combination of the just mentioned Val substitution coupled to a deletion of aa 106-131. Both rat and human CLEC9a appear to be absent aa 106-131 found in full-length mouse CLEC9a. Taking this into account, over aa 57-264, mouse CLEC9a shares 50% and 70% aa identity with human and rat CLEC9a, respectively.