Mouse CLEC9a Antibody

Catalog # Availability Size / Price Qty
AF6776
AF6776-SP
CLEC9a in Mouse Spleen.
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Mouse CLEC9a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CLEC9a in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CLEC9a, rhCLEC2, and rhCLEC12B is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CLEC9a
Lys57-Ile264
Accession # EDK99924
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CLEC9a in Mouse Spleen. View Larger

CLEC9a in Mouse Spleen. CLEC9a was detected in perfusion fixed frozen sections of mouse spleen using Sheep Anti-Mouse CLEC9a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6776) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC9a

CLEC9a (C-type lectin domain family member A; also DNGR-1) is a 50-52 kDa member of the group V C-type lectin domain containing family of receptors. In mouse, it is an activation receptor expressed on both CD8 alpha + CD24+ and plasmacytoid dendritic cells (DC). It assists in endocytosis but not phagocytosis, and appears to induce cytotoxic antitumor T cells by participating in class I MHC antigen presentation. CD8 alpha + DCs are believed to be particularly important for the disposal of dead cell material. Mouse CLEC9a is a 264 amino acid (aa) type II transmembrane glycoprotein. It contains a 35 aa cytoplasmic segment (aa 1-35) and a 208 aa extracellular region (aa 57-264) that possesses one C-type lectin domain (aa 144-256). CLEC9a forms disulfide-linked homodimers on the cell surface. There are multiple splice variants. The CLEC9a SwissProt entry (Q8BRU4) is 238 aa in length and shows a deletion of aa 106-131. Other isoforms contain either a 43 or a 67 aa substitution for aa 106-264, a Val substitution for 31-58, and a combination of the just mentioned Val substitution coupled to a deletion of aa 106-131. Both rat and human CLEC9a appear to be absent aa 106-131 found in full-length mouse CLEC9a. Taking this into account, over aa 57-264, mouse CLEC9a shares 50% and 70% aa identity with human and rat CLEC9a, respectively.

Long Name
C-type Lectin-like Receptor 9
Entrez Gene IDs
283420 (Human); 232414 (Mouse)
Alternate Names
CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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