Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3178P
Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody in Data
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Product Details
Citations (3)
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Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Coagulation Factor III in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Coagulation Factor III is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor
Ala29-Glu251
Accession # P20352
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Coagulation Factor III/ Tissue Factor in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Coagulation Factor III/ Tissue Factor in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3178P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
  3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
  4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
Entrez Gene IDs
2152 (Human); 14066 (Mouse)
Alternate Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

Product Datasheets

Citations for Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Alternative activation of human macrophages enhances tissue factor expression and production of extracellular vesicles
    Authors: PJ Hohensinne, J Mayer, J Kichbacher, J Kral-Point, B Thaler, C Kaun, L Hell, P Haider, M Mussbacher, JA Schmid, S Stojkovic, S Demyanets, MB Fischer, K Huber, K Wöran, C Hengstenbe, WS Speidl, R Oehler, I Pabinger, J Wojta
    Haematologica, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Androgen receptor dampens tissue factor expression via NF-?B and EGR1
    Authors: B Hoesel, M Mussbacher, B Dikorman, M Salzmann, A Assinger, L Hell, J Thaler, J Basílio, B Moser, U Resch, H Paar, N Mackman, JA Schmid
    J. Thromb. Haemost., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice
    Authors: Q Zhang, H Wang, H Li, J Xu, K Tian, J Yang, Z Lu, J Zheng
    Oncotarget, 2017;8(6):9488-9499.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody
By Anonymous on 11/03/2016
Application: Flow Sample Tested: IC-21 mouse macrophage cell line,Peritoneal macrophages Species: Mouse