Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse Coagulation Factor III in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Coagulation Factor III is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor
    Accession # P20352
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Coagulation Factor III/
Tissue Factor in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3178P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.

  • References:
    1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
    2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
    3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
    4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
  • Entrez Gene IDs:
    2152 (Human); 14066 (Mouse)
  • Alternate Names:
    CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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