Mouse Coagulation Factor III/Tissue Factor Antibody

Catalog # Availability Size / Price Qty
AF3178
AF3178-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Mouse Coagulation Factor III/Tissue Factor Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Coagulation Factor III in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Coagulation Factor III is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor
Ala29-Glu251
Accession # P20352
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA)
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line treated with LPS
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
  3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
  4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
Entrez Gene IDs
2152 (Human); 14066 (Mouse)
Alternate Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

Product Datasheets

Citations for Mouse Coagulation Factor III/Tissue Factor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Protease-activated receptor 2 protects against VEGF inhibitor-induced glomerular endothelial and podocyte injury
    Authors: Y Oe, T Fushima, E Sato, A Sekimoto, K Kisu, H Sato, J Sugawara, S Ito, N Takahashi
    Sci Rep, 2019;9(1):2986.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. The Myosin II Inhibitor, Blebbistatin, Ameliorates FeCl3-induced Arterial Thrombosis via the GSK3?-NF-?B Pathway
    Authors: Y Zhang, L Li, Y Zhao, H Han, Y Hu, D Liang, B Yu, J Kou
    Int. J. Biol. Sci., 2017;13(5):630-639.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells.
    Authors: Sebag S, Bastarache J, Ware L
    J Biol Chem, 2013;288(11):7875-84.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Elevated tissue factor expression contributes to exacerbated diabetic nephropathy in mice lacking eNOS fed a high fat diet.
    Authors: Li F, Wang CH, Wang JG, Thai T, Boysen G, Xu L, Turner AL, Wolberg AS, Mackman N, Maeda N, Takahashi N
    J. Thromb. Haemost., 2010;8(10):2122-32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Coagulation Factor III/Tissue Factor Antibody

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Mouse Coagulation Factor III/Tissue Factor Antibody
By Anonymous on 05/11/2018
Application: MSD assay Sample Tested: Serum and Plasma Species: Mouse

Used as detection antibody after labeling with Sulfo-Tag according to manufacturer's protocol (Meso Scale Diagnostics LLC)
Detection range - 8-30,000 pg/ml


Mouse Coagulation Factor III/Tissue Factor Antibody
By Anonymous on 04/17/2017
Application: Flow Sample Tested: Blood-derived monocytes Species: Mouse

Detection of Coagulation Factor III in Mouse mononuclear cells using Goat anti-Mouse F3 antibody (#AF3178, pink) at 2.5 ug/10^6 cells, or Isotype Control IgG (blue), followed by PE-conjugated anti-Goat secondary antibody.