Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Detection, FACS

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Coagulation Factor III/Tissue Factor Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor
Ala29-Glu251
Accession # P20352

Specificity

Detects mouse Coagulation Factor III in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Coagulation Factor III/Tissue Factor Antibody

Detection of Coagulation Factor III/Tissue Factor in Raw 264.7 cells treated with 1 µg/mL LPS overnight by Flow Cytometry

Raw 264.7 cells treated with 1 µg/mL LPS overnight were stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3178, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Coagulation Factor III/Tissue Factor Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: see below

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA)

Reviewed Applications

Read 4 reviews rated 5 using AF3178 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # http://www.rndsystems.com/product_results.aspx?k=3305-SE">3305-SE) under the conditions described in the Activity Assay Protocol.

References

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
  3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
  4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Gene Symbol

F3

UniProt

P20352

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Mouse Coagulation Factor III/Tissue Factor Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Coagulation Factor III/Tissue Factor Antibody

For research use only

Citations for Mouse Coagulation Factor III/Tissue Factor Antibody

Customer Reviews for Mouse Coagulation Factor III/Tissue Factor Antibody (4)

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4 Customer Ratings
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse Coagulation Factor III/Tissue Factor Antibody
    Name: Luiz Alexandre
    Application: Western Blot
    Sample Tested: Brain (caudate nucleus)
    Species: Mouse
    Verified Customer | Posted 10/21/2021
    This protein might be found in high levels in some conditions/tissues. If you calibrate a good concentration you will have a nice band around 50 KDa
    Mouse Coagulation Factor III/Tissue Factor Antibody AF3178
  • Mouse Coagulation Factor III/Tissue Factor Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreatic cancer cells
    Species: Mouse
    Verified Customer | Posted 09/15/2021
    Mouse Coagulation Factor III/Tissue Factor Antibody AF3178
  • Mouse Coagulation Factor III/Tissue Factor Antibody
    Name: Zoia Levashova
    Application: MSD assay
    Sample Tested: Serum and Plasma
    Species: Mouse
    Verified Customer | Posted 05/11/2018
    Used as detection antibody after labeling with Sulfo-Tag according to manufacturer's protocol (Meso Scale Diagnostics LLC) Detection range - 8-30,000 pg/ml
    Mouse Coagulation Factor III/Tissue Factor Antibody AF3178
  • Mouse Coagulation Factor III/Tissue Factor Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood-derived monocytes
    Species: Mouse
    Verified Customer | Posted 04/17/2017
    Detection of Coagulation Factor III in Mouse mononuclear cells using Goat anti-Mouse F3 antibody (#AF3178, pink) at 2.5 ug/10^6 cells, or Isotype Control IgG (blue), followed by PE-conjugated anti-Goat secondary antibody.
    Mouse Coagulation Factor III/Tissue Factor Antibody AF3178

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Protocols

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