Mouse Collectrin Alexa Fluor® 350-conjugated Antibody

Catalog #: AF4965U Datasheet / COA / SDS

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AF4965U-100UG

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Mouse Collectrin Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Collectrin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Collectrin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Collectrin
Glu15-Ile144
Accession # Q9ESG4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Collectrin

Collectrin (also Transmembrane protein 27) is a 37‑43 kDa member of the MEM27 family of proteins. It is expressed on pancreatic beta -cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208 amino acid (aa) type I transmembrane glycoptotein. It contains a 127 aa extracellular region (aa 15‑141) and a 60 aa cytoplasmic domain (aa 163‑222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26 kDa soluble fragment. Over aa 15‑144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.

Entrez Gene IDs

57393 (Human); 57394 (Mouse); 57395 (Rat)

Alternate Names

Collectrin; kidney-specific membrane protein; NX17; NX-17; TMEM27; transmembrane protein 27NX170610008J07Rik