Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Collectrin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Collectrin
Glu15-Ile144
Accession # Q9ESG4

Specificity

Detects mouse Collectrin in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human Collectrin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse Collectrin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Collectrin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collectrin

Collectrin (also Transmembrane protein 27) is a 37‑43 kDa member of the MEM27 family of proteins. It is expressed on pancreatic beta ‑cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208 amino acid (aa) type I transmembrane glycoptotein. It contains a 127 aa extracellular region (aa 15‑141) and a 60 aa cytoplasmic domain (aa 163‑222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26 kDa soluble fragment. Over aa 15‑144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.

Alternate Names

NX17, TMEM27

Entrez Gene IDs

57393 (Human); 57394 (Mouse); 57395 (Rat)

Gene Symbol

CLTRN

UniProt

Q9ESG4

Additional Collectrin Products

Product Documents for Mouse Collectrin Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Collectrin Biotinylated Antibody

For research use only

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FAQs

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