Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 620613
View all Complement Component C3aR Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived transfected with mouse C3aR
Accession # O09047

Specificity

Detects mouse C3aR in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Complement Component C3aR Antibody

Detection of Complement Component C3aR antibody in HEK293 Human Cell Line Transfected with Mouse Complement Component C3aRand eGFP antibody by Flow Cytometry.

Detection of Complement Component C3aR in HEK293 Human Cell Line Transfected with Mouse Complement Component C3aRand eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse Complement Component C3aR and eGFP was stained with (A) Rat Anti-Mouse Complement Component C3aR Monoclonal Antibody (Catalog # MAB10417) or (B) Rat IgG2a isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Complement Component C3aR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Mouse Complement Component C3aRand eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C3aR

Complement Component 3a receptor (C3a R) is an ~90 kDa G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Long Name

Complement Component C3a Receptor

Alternate Names

AZ3B, C3AR, C3AR1, C3R1, Complement Component C3a R

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Gene Symbol

C3AR1

UniProt

O09047

Additional Complement Component C3aR Products

Product Documents for Mouse Complement Component C3aR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Complement Component C3aR Antibody

For research use only

Citations for Mouse Complement Component C3aR Antibody

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