Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Complement Factor D/Adipsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor D/Adipsin
Ile26-Ser259
Accession # P03953

Specificity

Detects mouse Complement Factor D/Adipsin in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 1% cross-reactivity with rhGranzyme A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse Complement Factor D/Adipsin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Complement Factor D/Adipsin

Reviewed Applications

Read 1 review rated 5 using BAF5430 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor D/Adipsin

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids (aa) in length (aa 26‑259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa 26‑254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

UniProt

P03953

Additional Complement Factor D/Adipsin Products

Product Documents for Mouse Complement Factor D/Adipsin Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Complement Factor D/Adipsin Biotinylated Antibody

For research use only

Related Research Areas

Complement Pathway

Citations for Mouse Complement Factor D/Adipsin Biotinylated Antibody

Customer Reviews for Mouse Complement Factor D/Adipsin Biotinylated Antibody (1)

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  • Mouse Complement Factor D/Adipsin Biotinylated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Plasma
    Species: Mouse
    Verified Customer | Posted 08/23/2019
    Mouse Complement Factor D/Adipsin Biotinylated Antibody BAF5430

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