Mouse Complement Factor D/Adipsin Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Complement Factor D/Adipsin by Western Blot.
1 Image
Product Details
Citations (6)
Supplemental Products
Reviews (1)

Mouse Complement Factor D/Adipsin Antibody Summary

Species Reactivity
Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor D/Adipsin
Accession # P03953
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Complement Factor D/Adipsin, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Complement Factor D/Adipsin antibody by Western Blot. View Larger

Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids (aa) in length (aa 26‑259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa 26‑254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.

Entrez Gene IDs
1675 (Human); 11537 (Mouse); 54249 (Rat)
Alternate Names
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC; PFD; Properdin factor DADIPSIN

Product Datasheets

You must select a language.


Citations for Mouse Complement Factor D/Adipsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Adipsin promotes bone marrow adiposity by priming mesenchymal stem cells
    Authors: N Aaron, MJ Kraakman, Q Zhou, Q Liu, S Costa, J Yang, L Liu, L Yu, L Wang, Y He, L Fan, H Hirakawa, L Ding, J Lo, W Wang, B Zhao, E Guo, L Sun, CJ Rosen, L Qiang
    Elife, 2021;10(0):.
  2. Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction
    Authors: M Mizuno, B Khaledian, M Maeda, T Hayashi, S Mizuno, E Munetsuna, T Watanabe, S Kono, S Okada, M Suzuki, S Takao, H Minami, N Asai, F Sugiyama, S Takahashi, Y Shimono
    Cancers, 2021;13(16):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina
    Authors: D Pauly, D Agarwal, N Dana, N Schäfer, J Biber, KA Wunderlich, Y Jabri, T Straub, NR Zhang, AK Gautam, BHF Weber, SM Hauck, M Kim, CA Curcio, D Stambolian, M Li, A Grosche
    Cell Rep, 2019;29(9):2835-2848.e4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy
    Authors: X Wu, I Hutson, AM Akk, S Mascharak, CTN Pham, DE Hourcade, R Brown, JP Atkinson, CA Harris
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  5. Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina
    PLoS ONE, 2016;11(8):e0161898.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  6. Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis.
    Authors: Zou, Lin, Feng, Yan, Li, Yan, Zhang, Ming, Chen, Chan, Cai, Jiayan, Gong, Yu, Wang, Larry, Thurman, Joshua M, Wu, Xiaobo, Atkinson, John P, Chao, Wei
    J Immunol, 2013;191(11):5625-35.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Complement Factor D/Adipsin Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Mouse Complement Factor D/Adipsin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Mouse Complement Factor D/Adipsin Antibody
By Anonymous on 08/23/2019
Application: IP Sample Tested: Plasma Species: Mouse