Mouse CXCL3/GRO gamma /CINC-2/DCIP-1 Antibody

Catalog # Availability Size / Price Qty
AF5568
AF5568-SP
Chemotaxis Induced by CXCL3/DCIP‑1 and Neutral-ization by Mouse CXCL3/ DCIP‑1 Antibody.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Mouse CXCL3/GRO gamma /CINC-2/DCIP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL3/GRO gamma /CINC-2/DCIP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat CINC-2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL3/GRO gamma /CINC-2/DCIP-1
Ala28-Ser100
Accession # AAI17017
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL3/DCIP-1 (Catalog # 5568-CA)
Neutralization
Measured by its ability to neutralize CXCL3/DCIP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-3  µg/mL in the presence of 100 ng/mL Recombinant Mouse CXCL3/DCIP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CXCL3/DCIP‑1 and Neutral-ization by Mouse CXCL3/ DCIP‑1 Antibody. View Larger

Chemotaxis Induced by CXCL3/DCIP‑1 and Neutral-ization by Mouse CXCL3/ DCIP‑1 Antibody. Recombinant Mouse CXCL3/DCIP-1 (Catalog # 5568-CA) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL3/ DCIP-1 (100 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse CXCL3/ DCIP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5568). The ND50 is typically 0.5-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CXCL3 is also known as MIP-2 beta (macrophage inflammatory protein 2 beta), or DCIP-1 (dendritic cell inflammatory protein-1) in mouse, CINC2 (cytokine-induced neutrophil attractant 2) in rat, and GRO-gamma (growth-regulated oncogene gamma) in humans (1, 2). It is an 8 kDa proinflammatory member of the CXC subfamily of heparin-binding chemokines, also called alpha chemokines (1‑4). The Glu-Leu-Arg (ELR) motif near the CXCL3 N-terminus confers angiogenic properties and distinguishes it from interferon-inducible ELR- CXC chemokines, which are angiostatic (4). ELR+ and ELR- chemokines use CXCR2 and CXCR3 receptors, respectively (3, 4). Mature mouse CXCL3 shares 88% and 57% amino acid (aa) sequence identity with rat and human CXCL3, respectively. Among mouse ELR+ chemokines, it shares 82% aa sequence identity with CXCL2/GRO-beta /MIP-2 and 34% - 58% with CXCL1/GRO-alpha /KC, CXCL5/ENA-78 and CXCL7/NAP-2. Due to their similar sequence and activity, CXCL2 and CXCL3 are sometimes referred to collectively as CXCL2/3, but are separate gene products (4‑6). Mouse CXCL3 expression is induced in macrophages and early in maturation of DC by bacterial products such as lipopolysaccharides, and other inflammatory mediators (1, 7). It is chemotactic for CXCR2‑expressing neutrophils, helping to recruit them to areas of inflammation (1, 7). ELR+ chemokines also elicit endothelial cell chemotaxis, stimulating angiogenesis and playing a role in tumor development (3, 4). ELR+ chemokines upregulated by ischemia play a role in ischemia-reperfusion injury (5, 6). A decoy receptor, DARC (Duffy antigen receptor for chemokines) competes with CXCR2 for ELR+ chemokine binding, thus downregulating their effect (8). Neutrophil influx may also be downregulated by MMP-12, which has been found to inactivate CXCL3 and other ELR+ chemokines by cleaving them at the ELR site (9). Over aa 28-100, mouse CXCL3 shares 87.8% aa identity with rat CINC2.

References
  1. Nolan, K. F. et al. (2004) J. Immunol. 172:2201.
  2. Modi, W. S. and T. Yoshimura (1999) Mol. Biol. Evol. 16:180.
  3. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  4. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  5. Nesmelova, I. V. et al. (2008) J. Biol. Chem. 283:24155.
  6. Maheshwari, A. et al. (2004) Fetal Pediatr. Pathol. 23:145.
  7. Furuichi, K. et al. (2008) Front. Biosci. 13:4021.
  8. Takano, K. and H. Nakagawa (2001) Inflamm. Res. 50:503.
  9. Horton, L. W. et al. (2007) Cancer Res. 67:9791.
Entrez Gene IDs
2921 (Human); 330122 (Mouse); 171551 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3

Product Datasheets

Citation for Mouse CXCL3/GRO gamma /CINC-2/DCIP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CXCR4-dependent macrophage-to-fibroblast signaling contributes to cardiac diastolic dysfunction in heart failure with preserved ejection fraction
    Authors: N Zhang, Q Ma, Y You, X Xia, C Xie, Y Huang, Z Wang, F Ye, Z Yu, X Xie
    International journal of biological sciences, 2022;18(3):1271-1287.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse CXCL3/GRO gamma /CINC-2/DCIP-1 Antibody

Average Rating: 2 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
0%
2 Star
100%
1 Star
0%

Have you used Mouse CXCL3/GRO gamma /CINC-2/DCIP-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse CXCL3/GRO gamma /CINC-2/DCIP-1 Antibody
By Apsana Lamsal on 08/20/2018
Application: Western Blot Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Didnot give bands on specific lane, so many thick unspecific bands Performed western blot to see CXCL3 level, we have seen CXCL3 level is enhanced in 66CL4 from our RNA seq data. But the antibody gave so many unspecific bands and is hard to believe which one to choose. Do you have any images of the western blot performed for CXCL3?