Mouse CXCR2/IL-8 RB APC-conjugated Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 242216
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C6 rat glioma cell line transfected with mouse CXCR2
    Met1-Leu359
    Accession # P35343
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse CXCR2/IL‑8 RB APC‑conjugated Monoclonal Antibody (Catalog # FAB2164A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins
Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB APC‑conjugated Monoclonal Antibody (Catalog # FAB2164A). Quadrant markers were set based on control antibody staining (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR2/IL-8 RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

  • Long Name:
    Interleukin 8 Receptor B
  • Entrez Gene IDs:
    3579 (Human); 12765 (Mouse); 29385 (Rat)
  • Alternate Names:
    beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; IL-8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice.
    Authors: Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini P, Battaglia M, Allegretti M, Piemonti L
    Diabetes, 2015;64(4):1329-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
    EMBO J., 2012;31(17):3607-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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