Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 242216
View all CXCR2/IL-8RB Primary Antibodies »

Product Specifications

Immunogen

C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343

Specificity

Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CXCR2/IL-8RB APC-conjugated Antibody

Detection of CXCR2/IL-8 RB antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR2/IL-8 RB antibody in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A). Quadrant markers were set based on control antibody staining (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CXCR2/IL-8RB APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes and HEK293 human embryonic kidney cell line transfected with mouse CXCR2/IL-8 RB and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

P35343

Additional CXCR2/IL-8RB Products

Product Documents for Mouse CXCR2/IL-8RB APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCR2/IL-8RB APC-conjugated Antibody

For research use only

Citations for Mouse CXCR2/IL-8RB APC-conjugated Antibody

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