Mouse Cystatin C Antibody

Catalog # Availability Size / Price Qty
MAB1238
MAB1238-SP
Detection of Mouse Cystatin C by Western Blot.
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Mouse Cystatin C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse (rm) Cystatin C in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) Cystatin C is observed, but no cross-reactivity with rhCystatins A, D, S, SA or SN, rmCystatins B or EM, rhFetuin B, rmFetuin A, rhHPRG, or rhKininogen is observed.
Source
Monoclonal Rat IgG2B Clone # 207103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C
aa 21-140
Accession # P21460
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Cystatin C antibody by Western Blot. View Larger

Detection of Mouse Cystatin C by Western Blot. Western blot shows lysates of mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Cystatin C Monoclonal Antibody (Catalog # MAB1238) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is an inhibitor of cysteine proteases including Cathepsins B, H, K, L, and S. It is ubiquitous in mouse tissues with highest expression levels in brain.

Entrez Gene IDs
1471 (Human); 13010 (Mouse); 25307 (Rat)
Alternate Names
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; Cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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