Mouse Cystatin E/M Antibody

Catalog # Availability Size / Price Qty
MAB1284
MAB1284-SP
Product Details
FAQs
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Reviews (2)

Mouse Cystatin E/M Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cystatin E/M in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cystatin B, S, E/M, SA, S/N, recombinant mouse (rm) Cystatin A, C, D, rhFetuin A, B, rmHPRG, or rhKininogen is observed.
Source
Monoclonal Rat IgG2B Clone # 216820
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin E/M
Glu29-Val149
Accession # NP_082899
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cystatin E/M (Catalog # 1284-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cystatin E/M (Catalog # 1284-PI), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse Cystatin E/M (0.64 µg/mL, Catalog # 1284-PI) inhibition of Papain (0.1 µg/mL) cleavage of the fluorogenic peptide substrate Z-FR-AMC (100 µM, Catalog # ES009). The Neutralization Dose (ND50) is typically 7 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin E/M

Cystatin E/M is a member of family 2 of the cystatin superfamily (1 ‑ 6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (8). In addition to being a cysteine protease inhibitor, the human cystatin E/M is also a substrate for transglutaminases (9).

References
  1. Carninci, P. and Y. Hayashizaki (1999) Meth. Enzymol. 303:19.
  2. Carninci, P. et al. (2000) Genome Res. 10:1617.
  3. Shibata, K. et al. (2000) Genome Res. 10:1757.
  4. Kawai, J. et al. (2001) Nature 409:685.
  5. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
  6. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
  7. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
  8. Hong, J. et al. (2002) J. Neurochem. 81:922.
  9. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
Entrez Gene IDs
1474 (Human); 73720 (Mouse)
Alternate Names
CST6; cystatin 6; Cystatin E/M; cystatin M; cystatin M/E; Cystatin-6; cystatin-E; cystatin-M; cysteine proteinase inhibitor

Product Datasheets

FAQs

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Reviews for Mouse Cystatin E/M Antibody

Average Rating: 5 (Based on 2 Reviews)

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Mouse Cystatin E/M Antibody
By Anonymous on 03/20/2021
Application: This antibody works excellent. Sample Tested: Purified protein Species: Mouse

Mouse Cystatin E/M Antibody
By Anonymous on 02/21/2020
Application: WB Sample Tested: Purified protein Species: Mouse