Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Neutralization, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 216820
View all Cystatin E/M Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin E/M
Glu29-Val149
Accession # NP_082899

Specificity

Detects mouse Cystatin E/M in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cystatin B, S, E/M, SA, S/N, recombinant mouse (rm) Cystatin A, C, D, rhFetuin A, B, rmHPRG, or rhKininogen is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Cystatin E/M Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin E/M (Catalog # 1284-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Mouse Cystatin E/M (Catalog # 1284-PI)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Cystatin E/M (0.64 µg/mL, Catalog # 1284-PI) inhibition of Papain (0.1 µg/mL) cleavage of the fluorogenic peptide substrate Z-FR-AMC (100 µM, Catalog # ES009). The Neutralization Dose (ND50) is typically 7 µg/mL.

Reviewed Applications

Read 2 reviews rated 5 using MAB1284 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin E/M

Cystatin E/M is a member of family 2 of the cystatin superfamily (1 ‑ 6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (8). In addition to being a cysteine protease inhibitor, the human cystatin E/M is also a substrate for transglutaminases (9).

References

  1. Carninci, P. and Y. Hayashizaki (1999) Meth. Enzymol. 303:19.
  2. Carninci, P. et al. (2000) Genome Res. 10:1617.
  3. Shibata, K. et al. (2000) Genome Res. 10:1757.
  4. Kawai, J. et al. (2001) Nature 409:685.
  5. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
  6. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
  7. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
  8. Hong, J. et al. (2002) J. Neurochem. 81:922.
  9. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.

Alternate Names

CST6

Entrez Gene IDs

1474 (Human); 73720 (Mouse)

Gene Symbol

CST6

UniProt

NP_082899

Additional Cystatin E/M Products

Product Documents for Mouse Cystatin E/M Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cystatin E/M Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Customer Reviews for Mouse Cystatin E/M Antibody (2)

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2 Customer Ratings
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  • Mouse Cystatin E/M Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Mouse
    Verified Customer | Posted 03/20/2021
    Mouse Cystatin E/M Antibody MAB1284
  • Mouse Cystatin E/M Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Mouse
    Verified Customer | Posted 02/21/2020
    Mouse Cystatin E/M Antibody MAB1284

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