Mouse Dectin-2/CLEC6A alpha Isoform Antibody
Mouse Dectin-2/CLEC6A alpha Isoform Antibody Summary
Accession # Q9JKF4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Dectin-2, also known as CLEC6A alpha isoform, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 ( alpha isoform) is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3 - 6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 shares 71% aa sequence identity with human and bovine Dectin-2.
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- Kanazawa, N. et al. (2004) Immunobiology 209:179.
- Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
- Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
- Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
- Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
- McGreal, E.P. et al. (2006) Glycobiology 16:422.
- Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
- Aragane, Y. et al. (2003) J. Immunol. 171:3801.
Citation for Mouse Dectin-2/CLEC6A alpha Isoform Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Recognition of Aspergillus fumigatus hyphae by human plasmacytoid dendritic cells is mediated by dectin-2 and results in formation of extracellular traps.
Authors: Loures F, Rohm M, Lee C, Santos E, Wang J, Specht C, Calich V, Urban C, Levitz S
PLoS Pathog, 2015-02-06;11(2):e1004643.
Sample Types: Whole Cells
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