Mouse Dkk-2 Antibody

R&D Systems | Catalog # AF2435

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dkk-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dkk-2
Ser26-Ile259
Accession # Q9QYZ8

Specificity

Detects mouse Dkk‑2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Dkk-2 Antibody

Dkk‑2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Dkk‑2 in Mouse Embryo.

Dkk‑2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using 15 µg/mL Mouse Dkk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2435) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Dkk-2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Dkk‑2

Reviewed Applications

Read 1 review rated 5 using AF2435 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-2

Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators (1-3). Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain, which shows a configuration of cysteines conserved in prokineticin and colipase families, mediates Dkk-2 binding activities (2-4). The 226 amino acid (aa), ~35 kDa mature mouse Dkk-2 shares 41% and 34% aa identity with mouse Dkk-1 and Dkk-4, respectively. It also shares 99%, 96%, 96%, 96% and 94% aa identity with rat, human, canine, equine and bovine Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos (5). Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6 (3). When LRP6 is overexpressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling (6-8). However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes (9, 10). Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling (3). In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic (3). Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye. Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite downregulating Wnt antagonism in both cases (11, 12). Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition (11). In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density (12). Dkk-2-deficient mice are blind due to faulty differentiation of corneal epithelium (13).

References

  1. Monaghan, A.P. et al. (1999) Mech. Dev. 87:45.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Niehrs, C. (2006) Oncogene 25:7469.
  4. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
  5. Wu, W. et al. (2000) Current Biol. 10:1611.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Li, L. et al. (2002) J. Biol. Chem. 277:5977.
  8. Brott, B. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
  9. Mao, B. et al. (2002) Nature 417:664.
  10. Mao, B. and C. Niehrs (2003) Gene 302:179.
  11. Li, X. et al. (2005) Nat. Genet. 37:945.
  12. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  13. Mukhopadhyay, M. et al. (2006) Development 133:2149.

Long Name

Dickkopf-2

Alternate Names

Dkk2

Entrez Gene IDs

27123 (Human); 56811 (Mouse)

Gene Symbol

DKK2

UniProt

Q9QYZ8

Additional Dkk-2 Products

Product Documents for Mouse Dkk-2 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Dkk-2 Antibody

For research use only

Citations for Mouse Dkk-2 Antibody

Customer Reviews for Mouse Dkk-2 Antibody (1)

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  • Mouse Dkk-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 03/14/2017
    Mouse lung lysate probed with 1:1,000 DKK-2 Antibody showing two bands corresponding to fragments 60 kDa and 28 kDa. Buffer: 5% BSA in PBS. Secondary Ab: anti-Goat IgG 1:5,000.
    Mouse Dkk-2 Antibody AF2435

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