Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DPPII/QPP/DPP7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DPPII/QPP/DPP7
Asp34-Arg506
Accession # NP_114031

Specificity

Detects mouse DPPII/QPP/DPP7 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human DPPII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse DPPII/QPP/DPP7 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE)

Neutralization

Measured by its ability to neutralize Recombinant Mouse DPPII/QPP/DPP7 (0.02 µg/mL, Catalog # 3436-SE) cleavage of the fluorogenic peptide substrate KP-Amc (10 µM). The Neutralization Dose (ND50) is typically 0.45 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPPII/QPP/DPP7

Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have a high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The mouse DPP7 precursor consists of a  signal peptide (amino acids (aa) 1‑33) and a mature chain (aa 34‑506). The purified recombinant mouse DPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under otherwise identical conditions.

References

  1. Araki, H. et al. (2001) J. Biochem. 129:279.
  2. Maes, M.-B. et al. (2005) Biochem. J. 386:315.
  3. Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
  4. Lankas, G.R. et al. (2005) Diabetes 54:2988.

Long Name

Dipeptidyl-peptidase II

Alternate Names

DPP7, QPP

Entrez Gene IDs

29952 (Human); 83768 (Mouse); 83799 (Rat)

Gene Symbol

DPP7

UniProt

NP_114031

Additional DPPII/QPP/DPP7 Products

Product Documents for Mouse DPPII/QPP/DPP7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse DPPII/QPP/DPP7 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Mouse DPPII/QPP/DPP7 Antibody

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