Mouse DPPII/QPP/DPP7 Antibody

Catalog #: AF3436
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3436
AF3436-SP

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Mouse DPPII/QPP/DPP7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DPPII/QPP/DPP7 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human DPPII is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DPPII/QPP/DPP7
Asp34-Arg506
Accession # NP_114031

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Mouse DPPII/QPP/DPP7 (0.02 µg/mL, Catalog # 3436-SE) cleavage of the fluorogenic peptide substrate KP-Amc (10 µM). The Neutralization Dose (ND₅₀) is typically 0.45 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPII/QPP/DPP7

Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have a high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The mouse DPP7 precursor consists of a signal peptide (amino acids (aa) 1‑33) and a mature chain (aa 34‑506). The purified recombinant mouse DPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under otherwise identical conditions.

References

Araki, H. et al. (2001) J. Biochem. 129:279.
Maes, M.-B. et al. (2005) Biochem. J. 386:315.
Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
Lankas, G.R. et al. (2005) Diabetes 54:2988.

Long Name

Dipeptidyl-peptidase II

Entrez Gene IDs

29952 (Human); 83768 (Mouse); 83799 (Rat)

Alternate Names

carboxytripeptidase; Dipeptidyl aminopeptidase II; dipeptidyl arylamidase II; dipeptidyl peptidase 2; Dipeptidyl peptidase 7; Dipeptidyl peptidase II; dipeptidylpeptidase 7; dipeptidyl-peptidase 7; dipeptidyl-peptidase II; DPP II; DPP2; DPP7; DPPII; EC 3.4.14.2; QPP; Quiescent cell proline dipeptidase

Product Datasheets

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Related Research Areas

Citation for Mouse DPPII/QPP/DPP7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1